Objective: To investigate the practical problems of the application of a peptide retention model in the validation of peptide identification.
目的：解决肽段色谱保留时间预测模型在肽段鉴定结果验证中应用的实际问题。
Peptide mass fingerprinting analysis of proteins is the most widely used method for protein identification.
肽质谱指纹图分析是一种常用的蛋白质的鉴定方法。
To establish an improved procedure for isolation and identification of epitopes from a random peptide library displayed on the bacterial surface.
目的建立、改进并完善从细菌表面展示随机肽库中进行抗原表位筛选及鉴定的方法。
Conclusion: the application of "four-step extraction" method enhances the peptide recovery during in-gel digestion, and increases identification of the protein.
结论：应用“四步提取法”使得酶解肽段提取率和蛋白质的有效鉴定率明显提高。
Highly effective separation of complex peptide mixture is a prerequisite for protein identification with high coverage in proteomics.
复杂肽段混合物的有效分离是高覆盖率地鉴定蛋白质混合物的前提。
Highly effective separation of complex peptide mixture is a prerequisite for protein identification with high coverage in proteomics.
复杂肽段混合物的有效分离是高覆盖率地鉴定蛋白质混合物的前提。
应用推荐