As a screening tool, Slezak sees the LLMDA as occupying niche roles between PCR machines and sequencing.
作为一种检测工具，Slezak认为LLMDA将会在PCR与基因排序之间占有自己的一席之地。
We identified the geminivirus in Malvastrum coromandelianum from the molecular level by designing the primers, PCR, cloning and sequencing.
经设计通用引物、PCR扩增、克隆和测序，首次从分子水平鉴定了杂草赛葵上的双生病毒。
The specificity and reliability of the PCR were confirmed by sequencing the PCR products fragment.
产物片段的测序验证了PCR反应的可靠性。 为进口粮检疫中T 。
CONCLUSION: We first amplified mtDNA in circulating plasma from breast cancer patients by direct PCR, checked mutation by DHPLC and confirmed results by sequencing.
结论：本实验直接用PCR扩增乳腺癌血浆核酸中线粒体基因，并用DHPLC初步筛选突变并测序证实。
Mutation analysis was detected by direct sequencing RT-PCR products.
直接测序法检测RT PCR产物的基因变异；
Using genomic PCR and sequencing, FLT3/ITD mutation with or without chromosome translocation were examined in AML patients.
采用PCR联合序列检测伴有或不伴有染色体易位的急性髓性白血病患者FLT3基因突变情况。
Methods The mutation of mitochondrial DNA from all 18 family members of a chinese pedigree with maternally inherited aminoglycoside antibiotic-induced deafness was detected by PCR and DNA sequencing.
方法应用PCR和DNA序列分析技术，对一个有明确氨基糖苷类抗生素应用史的母系遗传耳聋家系共18人（包括聋人和听力正常者）的线粒体DNA进行研究。
Methods Genomic DNA was extracted from frozen tissues of 10 ameloblastomas and one malignant ameloblastoma. AMBN gene alterations were detected by PCR-direct sequencing.
方法收集10例成釉细胞瘤和1例成釉细胞癌的新鲜组织标本及相应外周血或牙龈黏膜组织，直接测序法分析AMBN基因；
Biochemical reaction, coagglutination test, metabolism inhibition test, polymerase chain reaction (PCR) assay, and DNA sequencing were employed to identify the isolated microorganisms.
阳性培养物用生化反应、协同凝集试验、代谢抑制试验、聚合酶链反应和DNA序列测定等方法进行鉴定。
The recombinants were analyzed and identified by restriction enzyme, PCR and sequencing.
通过酶切、PCR及测序鉴定各重组体。
Methods: PCR, gene cloning and successive sub cloning, DNA sequencing, prokaryotic temperature induction, etc. were used.
方法：采用PCR，克隆及连续亚克隆，序列分析，原核温度诱导表达等方法。
This paper reports a new method to identify the Chinese drug turtle shells using PCR product direct sequencing method.
用PCR产物直接测序法对中药材龟甲（板）进行鉴别。
Methods Genotype was characterized by PCR fingerprinting, intergenic spacer (IGS) sequencing, and phospholipase (PLB1) gene sequencing.
方法采用PCR指纹法、基因内间隔区（IGS）和磷脂酶基因（PLB1）测序分析鉴定基因型。
Using PCR-SSCP and PCR product direct sequencing techniques, we analysed the ORF region of SOX4 gene encoding in 15 lung cancer tissues and 8 normal controls.
本文采用PCR-SSCP及PCR产物直接测序等技术，对15例肺癌患者的组织标本及8例正常对照个体血细胞SOX4基因编码区进行了突变分析。
Design and search primers for multiple applications including PCR, DNA hybridization and sequencing.
设计和搜索多重引物，包括PCR引物、序列探针和测序引物。
PCR product was confirmed by DNA sequencing.
经过DNA测序证实该产物为目的扩增产物。
Methods: PCRbased cycle DNA sequencing is a method that combines PCR amplification with DNA sequencing.
方法：PCR循环测序法是将PCR扩增与核酸序列分析相结合的一种研究方法。
In addition to supporting conventional applications for PCR, hybridization and sequencing, NoePrimer provides advanced capabilities for multiplex PCR and high-throughput primer search and analysis.
它不但能进行常规PCR引物、杂交探针和测序引物的设计，同时，它还能进行多重pcr引物分析以及高通量的引物搜索和查找功能。
Methods Molecular biology technologies of RT-PCR, directed cloning and DNA sequencing were carried out.
方法采用RT -PCR、定向克隆和DNA测序等一系列分子生物学技术进行本实验。
Methods PCR-STR and DNA sequencing technology were used to detect rare alleles at 15 STR loci for 4650 unrelated individuals.
方法应用PCR -STR和DNA序列分析技术，对4650个无关个体在15个STR基因座中的罕见等位基因进行检测。
Genome DNA was extracted from peripheral blood, exon 1 and exon 2 of DAX1 gene were amplified by PCR for sequencing, and mutation screening of SF1 gene (exon 2-7) was conducted.
抽提外周血基因组dna，对DAX1基因2个外显子(外显子1和2)的PCR扩增产物进行测序分析；无突变者行SF 1基因(外显子2 ~ 7)突变筛查。
Methods Polymerase chain reaction(PCR)was used for 12 JAK2V617F -negative PV patients to amply the region of JAK2 exon 12, direct gene sequencing was performed to detect mutations of JAK2 exon 12.
方法采用聚合酶链反应（PCR）扩增12例JAK2V617F点突变阴性PV患者的JAK2外显子12片段，经基因测序与野生型JAK2外显子12比对，了解是否存在JAK2外显子12突变。
Sequence variation was screened by means of PCR single stranded conformation polymorphism (SSCP) and DNA sequencing.
用PCR -单链构象多态性(SSCP)和DNA序列分析方法确定扩增序列突变情况。
Mutation of VHL gene from tumor tissue was detected from tumor tissue by polymerase chain reaction (PCR) and direct sequencing.
采用单链聚合酶链反应(PCR)和测序法检测肿瘤组织中VHL基因的突变情况。
METHODS ITS1 and ITS2 genes of Pogostemon Cablin from different localities were identified by PCR direct sequencing.
方法采用PCR直接测序技术对不同产地的广藿香its1和ITS2基因进行测序分析。
By PCR-SSCP analysis, 17 PCR products were identified with different mobility of single strand DNA in propositus. 9 suspectable mutations were revealed with DNA sequencing analysis.
SSCP分析发现17个外显子pcr产物单链dna迁移率异常，通过DNA直接测序发现9个外显子可疑突变，但反向测序均未能证实。
On the basis of cloning and sequencing of OPF02 757 , two PCR primers were designed and a genome specific PCR marker for H.
在对OPF0 2 757进行克隆、测序的基础上，设计一对PCR引物，建立了簇毛麦基因组特异性PCR标记。
ITS sequences of ten kinds of Iris plants and an outgroup were obtained by primer design, PCR, gene cloning, sequencing and cluster analysis.
通过引物设计、P CR、基因克隆、测序，获得了10种鸢尾属植物和1个外类群种的ITS序列。
The results show that molecular identification based on PCR and direct sequencing is desirable to identify thrips species.
结果表明，基于PCR及直接测序技术的分子鉴定可以达到准确鉴定蓟马物种之目的。
The results show that molecular identification based on PCR and direct sequencing is desirable for identifying thrips species.
结果表明，基于PCR及直接测序技术的分子鉴定可以达到准确鉴定蓟马物种之目的。
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