The stored library was organized in two grades and 3-dimension structure, and a PCR screening system with high efficiency was built.
整个文库以两级3维的结构保存,建立了高效快速的PCR筛选系统。
", "how to write down a better calculating program which can provide me with clearer or more-in-detail information on our PCR screening results?
怎样编写一个更佳的计算程序来帮助我更清晰地掌握当前的PCR检测结果细节信息?
Methods PCR screening and DNA sequence were used to determine the drug resistance genes associated with class I integron in 4 clinical strains of P. aeruginosa.
方法采用PCR扩增、DNA测序、DNA序列比对的方法对4株铜绿假单胞菌临床菌株携带的I类整合子相关基因进行解析,采用接合试验对该类整合子进行定位分析。
Results and Conclusion: the PCR screening method was convenient and fast for confirming positive recombinant clone, and there was no need for preparation and purification of the plasmid.
结果和结论:以pcr方法筛查重组阳性克隆,可以简便快速鉴定重组阳性克隆,不需提取质粒。
As a screening tool, Slezak sees the LLMDA as occupying niche roles between PCR machines and sequencing.
作为一种检测工具,Slezak认为LLMDA将会在PCR与基因排序之间占有自己的一席之地。
Aim: To develop a PCR technique for rapid screening of recombinant plasmid in subtractive library of cDNA.
目的:消减文库构建过程中,用P CR技术快速筛选重组阳性克隆。
CONCLUSION: Because of the speediness, simpleness and good specificity, the PCR combined with restriction enzyme digestion can be used as a primary screening in the gene diagnosis of CMT1A.
结论:由于PCR -双酶切方法快速、简单、易操作,且特异性好,可作为CMT1A基因诊断的一种初筛方法。
Genome DNA was extracted from peripheral blood, exon 1 and exon 2 of DAX1 gene were amplified by PCR for sequencing, and mutation screening of SF1 gene (exon 2-7) was conducted.
抽提外周血基因组dna,对DAX1基因2个外显子(外显子1和2)的PCR扩增产物进行测序分析;无突变者行SF 1基因(外显子2 ~ 7)突变筛查。
Discrepant results confirm that PCR testing should be used as a screening tool rather than as a diagnostic tool.
对于有差异的结果证实,P CR检测应作为一个筛选工具,而不是作为一种诊断工具。
The PCR-Sequence gel silver staining was more rapid, immediate, simple and economy, which suits to the colony screening fragile X syndrome on a large scale.
运用PCR -序列分析胶银染法快速、直接、简便、实用,适合脆性X综合征的大规模群体筛查。
The genomic DNA PCR indicated that the genetic homogeneity between NIL-PL01 and NIL-APL01 reached 98.6%, which were being used in screening the molecular marker linked to apetalous trait.
利用随机引物对基因组DNA扩增结果表明,NIL -PL0 1和NIL -APL0 1之间多态性差异较小,遗传同质性达98.6 % ,该材料正用于筛选与无花瓣性状紧密连锁的分子标记。
A simple and rapid method for screening positive clones by PCR was also established in this paper.
并建立了快速、简便的PCR直接筛选和鉴定阳性克隆的方法。
Conclusion 3bs-pcr, a simple and economical method, could support us with an alternatively valuable assay for screening DNA point mutations from large number samples.
结论3BS - PCR方法操作简单经济,提供了有应用价值的大规模筛检dna点突变的方法。
Furthermore, PCR-ACRS was a fast and safe method for gene mutation screening.
另外,PCR -ACRS是一种快速、可靠的检测基因点突变的有效途径。
Furthermore, PCR-ACRS was a fast and safe method for gene mutation screening.
另外,PCR -ACRS是一种快速、可靠的检测基因点突变的有效途径。
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