Our method extended the utility of universal primers and provided implications for primer design and PCR amplification of some other genes.
新方法扩展了“通用引物”的适用范围，并为引物设计和一些其它基因的PCR放大提供了思路。
The optimization of reaction conditions and the primer design are the key factors for the success accomplishment of degenerate PCR.
成功进行简并PCR的关键是设计好两组引物库和对反应条件进行优化。
Here, a detailed protocol for bisulphite conversion, primer design, and optimization of PCR conditions is given.
这里，给出了亚硫酸氢盐转换，引物设计和PCR条件优化的一个详细的实验方案。
This review summarized the recently research process of Sq RT-PCR. It including extraction of total RNA, primer design, definition of cycle count, selection of inner reference and so on.
该文就半定量RT-PCR法的检测步骤及技术的关键因素（总RNA提取、引物设计、循环数的确定和内参的选择等）进行综述。
ITS sequences of ten kinds of Iris plants and an outgroup were obtained by primer design, PCR, gene cloning, sequencing and cluster analysis.
通过引物设计、P CR、基因克隆、测序，获得了10种鸢尾属植物和1个外类群种的ITS序列。
PCR-primer design is the most important step in polymerase chain reaction (PCR) technique.
引物设计是P CR技术中最重要的一环。
PCR-primer design is the most important step in polymerase chain reaction (PCR) technique.
引物设计是P CR技术中最重要的一环。
应用推荐