According to the bacteria gene sequence, we also designed PCR primer, and collected genome DNA for PCR rapid test, then tested and contrasted sequence.
根据菌属基因序列设计PCR引物，提取基因组dna进行PCR快速检测，然后进行测序比对。
The replicated stable results proved that two resistant genes could be identified simultaneously by using corresponding PCR primer under adaptable condition.
经反复验证，结果稳定准确，可用于在同一PCR反应体系中对两个抗病基因进行同时筛选鉴定。
Our method extended the utility of universal primers and provided implications for primer design and PCR amplification of some other genes.
新方法扩展了“通用引物”的适用范围，并为引物设计和一些其它基因的PCR放大提供了思路。
Objective: (1) to establish PCR reaction system that USES allele-specific primer PCR technique to detect SNP of KLOTHO gene.
目的：(1)建立使用等位基因特异性引物方法检测KLOTHO基因单核苷酸多态性的PCR反应体系。
Thisnovel protocol was named "single primer pre-incorporation PCR".
此程序被命名为“单引物预掺入pcr ”。
The optimization of reaction conditions and the primer design are the key factors for the success accomplishment of degenerate PCR.
成功进行简并PCR的关键是设计好两组引物库和对反应条件进行优化。
Conclusion This highly universal primer system in combinaition with highly specific hot initiated PCR might be used in the detection of medically important fungi in experimental or clinical specimens.
结论采用通用性强的引物系统配合特异性高的热启动PCR技术检测临床和实验室标本中是否存在病原性真菌的方法有重要的应用潜力。
Three pairs of oligonucleotide primer were used in triplex-PCR.
应用三对具有特异性的寡核苷酸引物，进行多重pcr扩增。
Method three pairs of oligonucleotide primer were used in triplex-PCR.
方法应用三对具有特异性的寡核普酸引物，进行多重PCR扩增。
Objective: to study and establish an allelic specific primer polymerase chain reaction (ASP-PCR) technique system and to apply this technique to study single nucleotide polymorphism (SNP) of genes.
目的：研究建立等位基因特异性引物pcr技术体系，并将其应用于基因单核苷酸多态性研究工作。
Single primer PCR was a method of chromosome walking to isolate sequences flanking a known DNA sequence with only one primer.
单引物pcr是一种只用一条引物就可以克隆已知序列侧翼未知区域的染色体步移方法。
This review summarized the recently research process of Sq RT-PCR. It including extraction of total RNA, primer design, definition of cycle count, selection of inner reference and so on.
该文就半定量RT-PCR法的检测步骤及技术的关键因素（总RNA提取、引物设计、循环数的确定和内参的选择等）进行综述。
Here, a detailed protocol for bisulphite conversion, primer design, and optimization of PCR conditions is given.
这里，给出了亚硫酸氢盐转换，引物设计和PCR条件优化的一个详细的实验方案。
The following work were done in this study: (1)TK and P32 gene of GTPV-TY were cloned by PCR with primer pairs designed by information published on GENBANK;
主要进行了以下工作：（1）根据GENBANK公布的羊痘病毒基因组序列设计引物，利用PCR克隆GTPV-TY株TK基因和P32基因；
A TC-RT-PCR method basing on reverse transcription with random primer facilitated the detection for those samples mixed infected by ASGV and ACLSV.
用随机引物反转录，通过TC-RT-PCR检测ACLSV和ASGV复合感染的梨样品也均获得了目标片段。
The positive bacteria strains were identified by random primer PCR.
随机引物pcr成功对阳性菌株进行了种属鉴定。
Conclusion: T. rubrum and T. mentagrophytes can be distinguished by PCR, (GACA)4 is the most suitable primer.
结论：红色毛癣菌和须癣毛癣菌可以用PCR方法加以鉴别，以（GACA）4作引物区分这两种菌较为合适。
ResultsThe PCR by specific flagellin gene primer has high sensitivity. All of 3 controls were negative.
结果用鞭毛蛋白基因作引物的PCR方法具有高灵敏度，其他3种对照结果阴性。
Specific primer PCR was a gene cloning technique with high efficiency, stability and accuracy.
特异引物PCR方法是一种高效、稳定、可靠的基因克隆手段。
ITS sequences of ten kinds of Iris plants and an outgroup were obtained by primer design, PCR, gene cloning, sequencing and cluster analysis.
通过引物设计、P CR、基因克隆、测序，获得了10种鸢尾属植物和1个外类群种的ITS序列。
Select the SSR primer whose colorant layer is located to do PCR augmentation to the monosome, and certificate the monosome according to the gained molecular label.
选择已经定位染色体的SSR引物对单染色体进行PCR扩增，根据得到的分子标记进行单染色体的鉴定。
In addition to supporting conventional applications for PCR, hybridization and sequencing, NoePrimer provides advanced capabilities for multiplex PCR and high-throughput primer search and analysis.
它不但能进行常规PCR引物、杂交探针和测序引物的设计，同时，它还能进行多重pcr引物分析以及高通量的引物搜索和查找功能。
Methods: PCR with an anchoring primer in IS 1541 and random primer;
方法：用一条锚定的IS 1541内部锚定引物和一条随机引物进行PCR扩增；
To establish a new method based on scorpion primer and PCR for rapid detection of the salmonellae in environmental water samples.
以蝎形引物pcr技术为基础建立一套环境水样沙门菌的快速检测方法。
Methods RNA of virus was obtained from samples of cerebrospinal fluid and serum and then gained with specific primer of Enterovirus through RT-PCR so as to detect specific gene fragment.
方法提取患者脑脊液和血清中的病毒rna，用肠道病毒特异性引物进行套式rt - PCR扩增，检测特异性基因片段。
The effect of different extraction buffers, different temperatures and different treatment times on the quality of DNA were compared by examining the DNA with random-primer PCR.
用随机引物pcr检测模板dna的质量，比较了不同提取缓冲液、不同温度及不同处理时间对模板dna质量的影响。
Primer is the key of specific reactions of PCR.
引物是PCR特异性反应的关键。
Particularly the method of microsatellite primer-PCR amplification makes clear the relationship of genetic diversity.
尤其是微卫星引物pcr扩增明确了桃蚜寄主生物型和体色生物型的遗传分化关系。
The PS-primer-based PCR assay provides a rapid and sensitive tool for the detection of P. sojae in soil and infected soybean tissue.
本套PCR检测技术为土壤及发病植株中大豆疫霉的检测提供了快速、灵敏的方法。
PCR-primer design is the most important step in polymerase chain reaction (PCR) technique.
引物设计是P CR技术中最重要的一环。
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