Individual genotype was detected by PCR method.
PCR方法检测个体基因型；
The P1 structure gene was amplified by PCR method.
采用PCR扩增方法获取P1结构基因。
PurposeThe aim is to obtain recombinant hirudin gene using PCR method.
目的应用PCR方法获得水蛭素基因。
Results PCR method can differentiate An. minimus A and C simply and quickly.
结果 经测序验证，PCR方法可以简单明确地将微小按蚊A、C加以鉴别。
Conclusion PCR method is better than isoenzyme analysis in differentiating An. minimus a and c.
结论对于微小按蚊a、C的鉴别，PCR方法明显优于同工酶方法。
Methods PCR method was applied to detect the KDR mutation of the target site (sodium channel) gene.
方法采用聚合酶链反应(PCR)方法，用自行设计的特异引物，对昆虫的钠离子通道基因进行扩增。
Objective: to establish a PCR method for gene identification and genotyping of clostridial neurotoxin.
目的：建立梭菌属神经毒素基因快速鉴定和分型的PCR方法。
Objective: To study the sensitivity of PCR method for the detection of Listeria monocytogenes in food.
目的：研究PCR检测不同食品中产单核李斯特菌的灵敏度。
After sequence analysis, RT PCR method was used to analyze the expression pattern in human fetal tissues.
序列分析后用RTPCR方法研究胎儿组织中的表达情况。
Objective To establish PCR method for the detection of the asymptomatic infection of Leishmania infantum.
目的建立适合检测我国婴儿利什曼原虫无症状感染的PCR方法。
Furthermore, the reaction system and program of ERIC-PCR method for the acid mineral drainage were optimized.
确定了ERIC方法用于酸性矿坑水样品时的最佳扩增体系和反应程序。
A chlamydia psittaci were isolated from affected chickens and the PCR method for chicken chlamydiosis was established.
本研究分离了鸡源鹦鹉热衣原体，建立了鸡衣原体病PCR诊断方法。
Methods Semi-quantitative PCR method was used in this research to estimate PERV copy-number of four mini-pigs in China.
方法采用半定量pcr方法检测了我国四种小型猪内源性逆转录病毒基因的拷贝数。
ConclusionDue to specificity on enterovirus, the RT PCR method has some value in the diagnosis of enterovirus infections.
结论该RT -PCR方法具有肠道病毒特异性，在肠道病毒感染的诊断中有一定应用价值。
It was proved that vitellogenin is synthesized in ovary and hepatopancreas in mature female shrimp through the RT-PCR method.
通过RT - P CR证实了雌虾的卵巢和肝胰腺是卵黄蛋白原的合成位点。
Methods: Expressions of WT1 and LRP genes were measured in 73 patients with acute leukemia and 23 normal controls by RT-PCR method.
方法：应用逆转录-聚合酶链反应(RT - pcr)法检测73例急性白血病患者及23例正常人的WT 1及LRP基因的表达。
The results of agarose gel electrophoresis and DNA sequence analysis indicated that the PCR method had high specificity and sensitivity.
结果经琼脂糖凝胶电泳和DNA序列测定证实，建立的PCR检测方法具有极高的灵敏度和较好的特异性。
All the 6 genotypes (9 strains) of measles virus showed DNA positive strand, it means this RT-PCR method is sensitive for measles viruses.
结果9株6种基因型麻疹病毒RT PCR均呈现阳性条带，说明本研究采用的RT PCR方法敏感。
Methods PCR method was used to detect micro-deletion in AZF gene in 62 oligospermatism and azoospermatism patients and 20 normal male controls.
方法 对62例无精症、少精症患者及20例正常男性采用多重聚合酶链反应法进行AZF区基因微缺失检测。
A TC-RT-PCR method basing on reverse transcription with random primer facilitated the detection for those samples mixed infected by ASGV and ACLSV.
用随机引物反转录，通过TC-RT-PCR检测ACLSV和ASGV复合感染的梨样品也均获得了目标片段。
METHODS: The regulating region of the human GCLC gene was cloned by PCR method to construct the wild type plasmid, which expressed luciferase reporting gene.
方法：通过PCR的方法克隆GCLC基因的部分转录调控区基因，构建表达虫荧光素酶报告基因的质粒；
With availability of a fragment of target DNA sequence, flanking unknown DNA sequence was obtained rapidly by successive chromosome walk using Il-PCR method.
运用该技术进行染色体连续步移，快速扩增DNA侧翼未知序列。
The single cell PCR method, which has been developed recently, has many advantages in HAB studies, especially for those species which are difficult to culture in the laboratory.
其中，单细胞PCR方法是对难以室内培养的有害赤潮藻种进行遗传特征研究的一项重要技术。
This research established a sensitive quantitative PCR method for SIV load testing. The creteria for accuracy judgement of duplication in quantitative PCR method was introduced.
本研究建立了敏感的SIV定量PCR检测方法，并提出了判断复孔检测结果是否准确的计算方法。
Objective to establish RT-PCR method for Puumala virus (PUUV) detection in order to confirm existence of Puumala virus in Jilin province and analyze gene sequence of Puumala virus.
目的建立检测普马拉病毒的RT - PCR方法，确认吉林省存在普马拉病毒，并对病毒的基因片段进行分析。
The aim of this assay is to investigate on the TaqMan Real-time PCR method for phytoplasma general detection which has the superiorities in saving time and preventing contamination.
本实验意在研究一种能够应用于植原体初筛，并且不易污染，易操作耗时短的通用方法。
Using the specific primers REA-FEA designed in this work by ourselves, we proved the specificity and sensitivity of the immuno-capture-PCR method again. The same result has been achieved.
利用本实验室自行设计的梨火疫病菌特异性引物REA-FEA，再次验证了免疫吸附-PCR技术的灵敏性和专化性，得到了同样的结果。
The applications of karyotyping and restriction fragment length polymorphism (RFLP) analyses, specific DNA probes and PCR method to the diagnosis of Candida albicans infections are reviewed.
综述了白色念珠菌感染的分子生物学诊断研究进展，包括核型分析、限制性片段长度多态性分析、特异的DNA探针及PCR等方法。
PCR method was applied to detect the proliferation condition and titer of PCV2 and to determine the harvest time of PCV2. The results showed that PCV2 could proliferate and passage on PK15 cells.
应用PCR方法检测组织细胞培养物中的病毒增殖和增殖滴度，测定了PCV2病毒收获时间，试验表明PCV2病毒在PK15组织培养可连续传代，增殖病毒。
The NPR1 gene was amplified from Arabidopsis thaliana genome DNA by DNA-PCR method. The DNA sequenced analysis showed that the sequence of amplified NPR1 gene was the same as the published sequence.
该文以DNA PCR扩增的方法，从拟南芥基因组DNA中克隆出NPR1基因，通过序列分析，所克隆的NPR1 基因与报道的基因序列完全一致。
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