On the basis of cloning and sequencing of OPF02 757 , two PCR primers were designed and a genome specific PCR marker for H.
在对OPF0 2 757进行克隆、测序的基础上，设计一对PCR引物，建立了簇毛麦基因组特异性PCR标记。
Use of most molecular marker systems depends on the polymerase chain reaction (PCR), which is an important technique for amplifying specific DNA sequences.
大多数分子标记体系的利用都取决于聚合酶链反应（PCR），这是一项扩增特定DNA序列的重要技术。
The results of PCR molecular detection, marker genes and foreign target-gene with insect-resistance were quite regular.
PCR分子检测与转化的标记基因和外源目的基因抗性三者极有规律性。
Conclusion: Using CK20 mRNA as a marker, the detection of micrometastasis with RT-PCR technique shows favorable specificity.
结论：应用rt - P CR法以CK20为标志物检测胃癌微转移细胞特异性高。
Methods Paired blood and RB tumor samples from 16 patients were analyzed with fluorescent PCR for LOH at 14 microsatellite marker loci on chromosome 13.
方法16个RB患者成对的肿瘤与其相应血清标本在13号染色体上14个微卫星标记处通过荧光PCR进行扩增，分析测定LOH ；
Conclusion DNA marker can be produced for laboratory use with RD-PCR technology, which is a simple, efficient and inexpensive method for experiment.
结论采用限制性差异显示技术制备DNA标记物，不仅操作简便、快捷、提高工作效益，且大大降低费用，更适合实验室实际需要。
The results from this study indicate that the use of backcrossing with a PCR-based DNA marker was useful in waxy wheat breeding. These partial waxy wheat lines can be used in field production.
研究表明采用回交转育与分子标记辅助选择相结合的方法，是培育农艺性状优良部分糯性小麦的一种非常有效的方法。
PCR analysis indicated that HPT marker gene has been deleted successfully.
PCR扩增检测杂交单株，结果显示，标记基因得到有效剔除。
The genomic DNA PCR indicated that the genetic homogeneity between NIL-PL01 and NIL-APL01 reached 98.6%, which were being used in screening the molecular marker linked to apetalous trait.
利用随机引物对基因组DNA扩增结果表明，NIL -PL0 1和NIL -APL0 1之间多态性差异较小，遗传同质性达98.6 % ，该材料正用于筛选与无花瓣性状紧密连锁的分子标记。
Methods: D10S1265, a fluorescent labeled polymorphic microsatellite marker, was analyzed in 83 cases of sporadic CRC and normal tissue DNA by PCR.
方法：荧光标记的多态性微卫星引物d10s1265与83例结直肠癌的肿瘤和正常组织进行PCR反应。
Methods were amplified PCR technology for different sizes of the specificity of the band, its concentration, in proportion to the needs of mixed composition of the DNA marker.
方法运用PCR技术分别扩增不同大小的特异性条带，确定其浓度，按比例混合后组成所需要的DNA分子量标准。
Conclusion of PCR method can be an excellent DNA marker.
结论P CR扩增法可以获得优良的DNA分子量标准。
Conclusion of PCR method can be an excellent DNA marker.
结论P CR扩增法可以获得优良的DNA分子量标准。
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