Prove RT-PCR detection method is peculiar very high.
结果表明RT-PCR检测方法有很好的特异性。
The results completely matched the conventional PCR detection results.
结果与常规PCR方法检测结果完全相符。
The positive ratio of the seedling is 44.7% by GUS detection and 52.6% by PCR detection.
对再生植株进行GUS检测发现有44.7%植株呈阳性，PCR检测的结果也显示有52.6%的植株为阳性植株。
The PCR detection of bovine, sheep and goat derived materials was optimized using beef and goat meat.
以牛肉、山羊肉为试验材料，对动物产品及饲料中牛源、羊源性成分PCR检测方法进行了优化。
TTV infection in the patients was retrospectively analyzed with nested PCR detection of TTV DNA in serum.
采用套式聚合酶链反应方法对合并TTV感染情况进行回顾性研究。
The PCR detection proves the sequnece has successfully recombined with the genome of Arabidoposis thaliana.
PCR检测证明，目的基因已成功导入模式植物拟南芥中；
The multiplex PCR is a progressing rapid detection method developed from traditional PCR detection technique.
多重PCR方法是建立在传统PCR检测技术基础上的快速简便的检测手段。
Simultaneously, the result of RT-PCR detection confirmed the target gene has been expressed on the level of transcription.
用RT-PCR 技术检测表明，目的基因在转化再生植株中能够正常转录。
So, the traditional and widely used Serology and PCR detection method can no longer meet the demands to fast and simultaneous detect lily viruses.
目前应用最广泛的血清学、PCR等传统的检测技术已不能完全满足大量、快速对百合病毒病检测的要求。
PCR detection results showed that 30 plants showed positive in 37 kanamycin resistance plants. Its indicated that the foreign gene VP7 had already integrated into genome of receptor plants.
P CR检测结果表明，37株卡那霉素抗性植株中30株为阳性，说明vp7外源基因已整合到受体植物基因组中。
CBF1 gene from rape was transformed into Arabidopsis thaliana by Agrobacterium tumefaciens-mediated method to screen its transgenic plants with resistance and conduct PCR detection and GUS staining.
采用根癌农杆菌介导法将油菜CBF1基因转入拟南芥，筛选抗性转基因拟南芥植株，进行PCR检测和GUS染色。
Polymerase chain reaction-based (PCR-based) and enzyme-linked immunoabsorbent assay (ELISA) methods are now applied in the detection of major food-borne pathogens.
依据聚合酶链反应(PCR)和酶联免疫吸附检测(ELISA)的方法目前被应用于主要食源性致病菌的检测。
Viral detection by reverse transcription polymerase chain reaction (RT-PCR) assay, and.
采用逆转录聚合酶链式反应(RT - PCR)检测病毒。
Current multiplex polymerase chain reaction (PCR) techniques can at most offer detection from among 50 organisms in one test.
现行的多元聚合酶链反应(PCR)技术最多只能在50种生物内进行一次检测。
Results it was found that DNA fixation method had a great influence on detection of PCR products of M. tuberculosis. Alkali fixation had a good sensitivity and reproducibility.
结果结核分枝杆菌dna固定方法对P CR扩增产物的检测结果具有较大的影响，碱液固定具有较好的敏感性和重复性。
Methods: Polymerase chain reaction (PCR) was adopted in detection of DNA of mycobacterium tuberculosis from peripheral blood and sputum in 64 tuberculosis patients.
方法：采用聚合酶链反应(PCR)技术，检测了64例肺结核患者的外周血与痰标本结核杆菌dna。
The result indicated that fluorogenetic quantitation RT-PCR may be used as a good reference in detection of H5N1 TIV.
这说明荧光定量RT-PCR方法可以对临床上H5N1虎源流感病毒检测提供参考。
Conclusion Comprehensive methods should be applied to increase the positive rate of PCR in the detection of variable region.
结论应综合采用多种方法以提高基因高突变区PCR检测阳性率；
The elementary procedure of this technology contains sample disposal, protein enzyme digestion, in-situ PCR and the detection of sequence copied.
原位PCR技术的基本步骤包括标本处理、蛋白酶消化、原位PCR扩增和扩增后产物的检测。
Objective: To set up a rapid and simple PCR assay for Candida detection and identification by using internal tran scribed spacer (ITS) universal primers.
目的：应用内转录间隔区(its)通用引物建立一种较为快速、简便的检测和鉴定念珠菌的聚合酶链反应(PCR)方法。
The results of PCR molecular detection, marker genes and foreign target-gene with insect-resistance were quite regular.
PCR分子检测与转化的标记基因和外源目的基因抗性三者极有规律性。
So the PCR method was suitable for rapid and sensitive detection of human botulism borne clostridia.
因此，此P CR方法可用于快速敏感地检测引起人类肉毒中毒的梭菌。
Objective To evaluate the value of PCR-probe hybridization-ELISA for detection of mycobacterium tuberculosis(M. tuberculosis).
目的评价聚合酶链反应探针杂交酶显色方法，检测结核分支杆菌。
The methods of detecting AI include mainly serum-testing and molecular biologic methods depending on PCR, of which nucleic acid testing techniques depending PCR belongs to new detection methods.
禽流感的检测大体可以分血清学检测和依赖于PCR技术的分子生物学检测，其中依赖核酸的扩增检测技术属于新型检测手段。
Aim: To evaluate the clinical value of MSP (methylation specific PCR) in detection of tumor suppressor genes methylation in the colorectal cancer.
目的：评价特异性甲基化pcr (msp)分析法检测抑癌基因高甲基化对结、直肠癌的诊疗价值。
Methods: Seafoods were collected randomly from different hotels in Nanjing and EHEC O157: H7 was detected and regular biochemistry, serology and virulent genes detection by PCR were carried out.
方法：随机采取南京市各大宾馆的海水产品，进行大肠埃希菌o 157：H7的检测，进行常规生化和血清学、毒力基因的PCR检测。
Objective To investigate the clinical application of real time fluorescence quantitive Polymerase Chain Reaction (FQ-PCR) in detection of HCV RNA in serum and peripheral blood monocular cells (PBMC).
目的探讨实时荧光定量聚合酶链反应(FQ - PCR)在检测外周血清及单核细胞中hcvRNA含量的临床应用价值。
Based on the gene sequence of circumsporozoite protein(CSP) of Plasmodium vivax , the PCR/DNA probe detection system was prepared.
根据间日疟原虫环子孢子表面蛋白（CSP）基因序列研制了PCR／DNA探针检测系统。
So this semi-nested RT-PCR could be used for rapid detection of the muscovy duck reovirus disease.
因此，该半套式RT-PCR可以用于番鸭呼肠孤病毒病的临床快速检测。
Objective To develop a PCR assay for the rapid and specific detection of Proteus mirabilis.
目的建立一种检测奇异变形杆菌快速且特异的PCR方法。
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