Methods STK15 gene amplification in 40 cases carcinoma tissues and normal tissues as control was detected by differential PCR approach.
方法应用差异PCR方法检测40例喉鳞状细胞癌及癌旁正常对照组织STK15基因扩增的情况；
Methods: PCRbased cycle DNA sequencing is a method that combines PCR amplification with DNA sequencing.
方法：PCR循环测序法是将PCR扩增与核酸序列分析相结合的一种研究方法。
Methods The telomerase activity was detected by TRAP( telomeric repeat amplification protocol) -ELISA which based upon PCR amplification of the initial telomerase product and detected by ELISA.
方法采用端粒重复序列扩增文件-酶标法（TRAP-ELISA）测定尖锐湿疣皮损中端粒酶的活性，并与正常皮肤和恶性肿瘤对照比较。
Methods Choose correct and effective PCR cycle number according to standard plasmid's amplification curve in real-time PCR;
方法根据标准质粒荧光定量PCR的扩增曲线选择合适有效的扩增循环次数；
Objective To construct the recombinant plasmid that highly expressed human zona pellucida 3. Methods The techniques of PCR amplification, T-A vector ligation, and sub-clone were used.
目的：构建高效表达人透明带蛋白3的真核重组表达载体。方法：利用PCR、T- A载体克隆和亚克隆等技术。
Objective To construct the recombinant plasmid that highly expressed human zona pellucida 3. Methods The techniques of PCR amplification, T-A vector ligation, and sub-clone were used.
目的：构建高效表达人透明带蛋白3的真核重组表达载体。方法：利用PCR、T- A载体克隆和亚克隆等技术。
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