Methods Enzyme digestion was performed to isolate single HERG-HEK cell and whole-cell patch-clamp technique was used to record HERG current and kinetic curves.
方法传代得到单个HERG-HEK细胞,应用全细胞膜片钳技术记录HERG-HEK细胞上的HERG钾电流和动力学曲线(激活、失活、复活和去活化)。
Patch all 3 curves below and use a span count of 2 in both directions.
修补下面所有3条曲线,在各个方向上的跨度为2。
Results: (1) Whole-cell patch-clamp recording technique was established, and highly stable whole cell ion channels' current curves of normal ventricular myocytes were ploted.
结果:(1)建立了膜片钳全细胞记录技术平台,获得了高稳定性的正常心室肌细胞全细胞离子通道电流图。
Methods Enzyme digestion was performed to isolate single HERG-HEK cell and whole-cell patch-clamp technique was used to record HERG current and kinetic curves.
膜片钳技术是一种以记录离子通道的离子电流来反映细胞膜上单一的或多个的离子通道分子活动的技术。
Only a single melting or cooling peak was found in DSC curves for all blends, showing cocrystalline in patch-crystal level.
通过DSC上(熔融峰、结晶峰)呈现单峰确定了此共混体系在片晶水平上共晶;
The 3D surface can be defined directly by the boundary curve of the patch surface. The surface will be C1 continuous if the boundary curves connecting each two patches are C1 continuous.
利用曲面网格的边界曲线直接定义3D曲面,既能保证曲面的光滑过渡,即C1连续,又可避免通过确定扭矢来达到控制曲面的复杂计算。
The 3D surface can be defined directly by the boundary curve of the patch surface. The surface will be C1 continuous if the boundary curves connecting each two patches are C1 continuous.
利用曲面网格的边界曲线直接定义3D曲面,既能保证曲面的光滑过渡,即C1连续,又可避免通过确定扭矢来达到控制曲面的复杂计算。
应用推荐