METHODS: Whole cell patch-clamp recording techniques.
方法：全细胞膜片箝技术。
The automatic patch-clamp system had been analyzed and modeled by using the method of UML in RUP.
通过基于RUP的UML系统建模方法对全自动膜片钳软件系统进行了分析和建模。
The whole-cell patch-clamp recording technique was used to record the change of sodium channel current influenced by carvedilol.
方法用急性酶解法获得单个大鼠心室肌细胞，标准的全细胞膜片钳技术记录钠通道电流。
Methods Enzyme digestion was performed to isolate single HERG-HEK cell and whole-cell patch-clamp technique was used to record HERG current and kinetic curves.
方法传代得到单个HERG-HEK细胞，应用全细胞膜片钳技术记录HERG-HEK细胞上的HERG钾电流和动力学曲线（激活、失活、复活和去活化）。
Results: (1) Whole-cell patch-clamp recording technique was established, and highly stable whole cell ion channels' current curves of normal ventricular myocytes were ploted.
结果：(1)建立了膜片钳全细胞记录技术平台，获得了高稳定性的正常心室肌细胞全细胞离子通道电流图。
The routine controls and inspects the patch-clamp amplifier by employing the personal computer and data acquisition, and then evaluates the analog parameters using relative arithmetic.
利用计算机和参数控制与数据采集器对膜片钳放大器进行数字控制和参数检测，并采用最小二乘拟合等算法计算相关参数。
The first one clearly elucidates the operational principle of patch-clamp amplifier and the compensatory technology, which are the fundamental for fulfilling the automation of patch clamp.
首先对膜片钳放大器工作原理及补偿技术进行了深入研究，这是实现膜片钳自动化的基础。
To accomplish the automatic patch-clamp system controlled by computer system, to realize the automatization during experiment, the software system of automatic patch-clamp should be designed.
为了完成由计算机系统控制的全自动化膜片钳，实现整个实验过程的自动化，需要设计全自动膜片钳的软件系统。
Using the whole-cell patch-clamp technique, clear evidence was provided for the existence of a hitherto unreported, voltage-dependent nonselective cation current (NSCC) in rabbit ventricular myocytes.
应用全细胞膜片钳制技术，我们首次报道了在兔的心室肌细胞存在一种电压依赖性的非特异性阳离子流（NSCC）。
METHODS Patch clamp technique with whole cell configuration.
方法膜片钳制技术全细胞记录模式。
METHODS The patch clamp whole cell recording technique was used.
方法采用膜片钳全细胞式记录技术。
Patch clamp technique is a kind of technique which reflecting membrane molecular activity of one or more ion channel by record ion current of ion channel.
膜片钳技术是一种以记录离子通道的离子电流来反映细胞膜上单一的或多个的离子通道分子活动的技术。
The patch clamp technique has become the main method for cell electrophysiology research.
膜片钳技术已成为细胞电生理学研究的重要手段。
Methods: The potassium currents of rat hippocampal neurons in primary culture were measured with patch clamp whole cell recording.
方法：膜片钳全细胞记录测量原代培养大鼠海马神经元膜的钾离子电流。
Methods: Patch clamp whole cell recording technique was used.
方法：膜片钳全细胞电流记录方法。
The patch clamp technique has become the main method for the molecule and cell research.
膜片钳技术已成为分子和细胞学研究的重要手段。
The widely using of patch clamp technique has greatly promoted our knowledge of ion channels.
膜片钳技术的广泛应用极大推动了人们对离子通道的认识。
Methods We used the improved enzymatic dissociation method and the patch clamp whole cell recording.
方法采用改进的酶解分离法分离单个细胞并经膜片钳全细胞记录法进行鉴定。
METHODS Voltage gated potassium outward current was recorded using whole cell patch clamp techniques in the cultured rat hippocampal neurons.
方法在培养的大鼠海马神经元上用膜片钳全细胞记录钾通道电流。
Methods: The patch clamp was used to record the changes in average whole cell current and the conductance of GABA-A receptor on cultured hippocampal neurons in different developing stages.
方法用膜片钳记录仪在体外培养的不同时期，记录大鼠海马神经元GABA-A受体离子通道全细胞通道电流、电导的变化。
METHOD The whole cell patch clamp techniques were used.
方法采用全细胞膜片钳技术。
Methods: the whole cell patch clamp technology was used to record this current and study its change under the condition of hypoxia-reoxygenation model.
方法：运用全细胞膜片钳技术记录持续性钠电流，并观察其在缺氧-复氧模型下的变化。
Conclusion a simple and fast method for acutely isolating rats cortex neurons suitable for patch clamp is successfully found.
结论成功建立了一种简单快速的适用于膜片钳技术的大鼠皮层神经元急性分离改良方法。
Method The whole cell patch clamp method was used.
方法应用全细胞膜片钳制技术。
Methods: Whole-cell patch clamp technique was used to determine sodium channel cur rent.
方法：采用全细胞膜片钳技术，记录钠通道电流。
Methods the chloride channel currents were recorded in cultured A549 cells by using the whole-cell mode of patch clamp techniques.
方法在离体培养的A549细胞上，用膜片钳全细胞模式记录氯通道电流。
METHOD Whole cell patch clamp techniques.
方法全细胞膜片箝技术。
The first step of recording calcium channel current of cardiomyocytes with patch clamp technique is dissociation of myocytes.
用膜片箝技术记录心肌细胞钙通道电流，首先必须获得单个心肌细胞。
The first step of recording calcium channel current of cardiomyocytes with patch clamp technique is dissociation of myocytes.
用膜片箝技术记录心肌细胞钙通道电流，首先必须获得单个心肌细胞。
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