In some orchids the velamen includes spongy and fibrous bodies near the passage cells.
在一些兰科植物里面,根被在通道细胞附近包含有海绵状的和纤维状的物体。
Finally, primary cells from tracheal aspirates behaved in an identical manner as later passage cells.
最后,气管抽吸的原代细胞和之后的传代细胞有同样模式。
Even in the 128th CSFV39-PK15 passage cells, we still could detect the existence of CSFV39. Both immunofluorescence antibody test and RT-PCR assay showed positive results.
即使在连续传至128代的CSFV39 - PK 15传代细胞中,CSFV仍持续存在:呈免疫荧光抗体反应阳性和RT - PCR检测阳性。
After all, both cancer cells and some very simple forms of life appear highly resistant to the passage of time.
毕竟时间的流逝对癌细胞和一些简单的生命形态就毫无作用。
Endodermis the innermost part of the cortex, consisting of a single layer of cells that controls the passage of water and solutes between the cortex and the stele.
内皮层:是皮层的最内层,仅有一层细胞,可以控制皮层和中柱之间水和溶质的通过。
After primary culture, rabbit corneal epithelium and endothelium were digested and inoculated in bottle having hFLP feeder cells for serial passage.
兔角膜上皮和内皮细胞组织块原代培养,消化接种于人胚肺饲细胞瓶传代培养。
Results Flow cytometry analysis showed that fetal bone marrow derived MSC exhibited the characteristics of mesenchymal cells, MSC had an active proliferative ability in primary and passage cultures.
结果流式细胞术证明,MSC具有间充质细胞的特征。原代及传代培养显示,胎儿骨髓msc具有活跃增殖的能力。
Objective To study the process of aging and its potential mechanisms during passage of vascular endothelial cells.
目的探讨血管内皮细胞在传代过程中的衰老过程及机制。
Methods: The advantage of serum free and clone culturing technology was performed to isolate, culture and passage neural stem cells from embryonic rat hippocampus, striatum and spinal cord.
方法:利用无血清培养和细胞克隆培养技术,从胚胎大鼠海马、纹状体、脊髓等区分离神经干细胞,进行体外扩增培养、传代、贴壁分化观察。
Conclusion This study suggests a valid and safe method that can be used for efficient passage of neural stem cells.
结论消化振荡法对人胚胎神经干细胞的活性影响较小,是一种有效、安全的将神经球打散成单细胞悬液的实验方法。
The advantage of serum free and clone culturing technology was performed to isolate, culture, passage and induce neural stem cells from embryonic rat cortex, hippocampus and striatum.
方法从胚胎大鼠的大脑皮质、海马、纹状体等组织分离神经干细胞,用无血清培养技术在体外进行培养、扩增、传代和诱导分化。
Result Cobblestone-like submandibular gland acinar cells were found after purification, and amylase was successfully detected both in culture medium and cells of passage 0-5 respectively.
结果纯化后的颌下腺腺泡细胞呈铺路石样形态,原代至第5代培养基及细胞内均可检测到淀粉酶表达;
Cultured cells could stably grew and passage.
培养的细胞可以稳定生长传代。
Furthermore, it was found that the cultured cells recovered their ability to grow when the substrate was reused every passage.
进一步研究表明,每次传代时重复使用基质能够使所培养的细胞恢复原来的活力。
Conclusions RCC cells can be amplified by passage Culture, but only within the cells of four generations.
结论软骨细胞可以通过传代培养获得扩增,但仅限于四代以内细胞。
Up to the sixth passage, the cells lost their proliferation ability.
至第六代,细胞已失去增殖能力。
After more than 4 months of passage (approximately 26 passages), the cells still retained their characteristics.
在传代4个月后(约26代),细胞仍然保持了其生物学特性。
Cultured pulmonary trunk wall cells at the 2nd to 3rd passage were applicable to investigations of hypoxic experiments and efficiency of drugs.
培养的第2~3代细胞适用于缺氧实验和药物作用机制的研究。
To study the process of aging and its potential mechanisms during passage of vascular endothelial cells.
目的 探讨血管内皮细胞在传代过程中的衰老过程及机制。
Methods PC12 cells and BV2 cells, instead of neurons and microglia separately, were co-cultivated in transfer screen by cell passage method.
方法采用细胞株传代法培养PC12细胞及BV2细胞,分别替代神经元细胞和小胶质细胞;
PCR method was applied to detect the proliferation condition and titer of PCV2 and to determine the harvest time of PCV2. The results showed that PCV2 could proliferate and passage on PK15 cells.
应用PCR方法检测组织细胞培养物中的病毒增殖和增殖滴度,测定了PCV2病毒收获时间,试验表明PCV2病毒在PK15组织培养可连续传代,增殖病毒。
The adherent cells were preserved to passage, the purity of ADSCs was analyzed by immunocytochemistry method, and cell growth was observed, then proliferation capability and cell cycle were detected.
方法使用密度梯度离心法分离不同年龄段脂肪间充质干细胞进行培养,保留贴壁细胞传代,分析脂肪间充质干细胞的纯度,观察细胞生长情况,检测其增殖活性、细胞周期。
However, the activity of the seventh passage of cells was significantly decreased, and the survival rate was only 78%.
至第7代细胞活力呈明显下降趋势,细胞成活率仅为78%。
Passage of superparamagnetic iron oxide labeled rat bone marrow mesenchymal stem cells in vitro: Do iron particles change with passage?
超顺磁性氧化铁体外标记大鼠骨髓基质干细胞的传代:细胞中铁粒子会随传代而变化吗?
Results Population doubling time of corneal stromal cells prolonged significantly since passage 7. MTT assay also showed cell viability of passage 7 decreased to 60% of passgage 1 (P< 0. 01 ).
细胞群体倍增时间由第7代起显著延长(P<0.01);MTT比色法的结果亦显示,第7代细胞的增殖能力开始大幅下降(P<0.01)。
Results Population doubling time of corneal stromal cells prolonged significantly since passage 7. MTT assay also showed cell viability of passage 7 decreased to 60% of passgage 1 (P< 0. 01 ).
细胞群体倍增时间由第7代起显著延长(P<0.01);MTT比色法的结果亦显示,第7代细胞的增殖能力开始大幅下降(P<0.01)。
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