Methods: Genes were spliced by overlap extension.
方法：采用重叠区扩增基因拼接法。
Conclusion Splicing overlap extension by PCR is a convenient method to obtain recombinant gene.
结论：重叠延伸pcr是一种获得重构基因的简捷方法。
The overlap extension mediated by restriction endonuclease to obtain the full length gene was established.
建立一种用于克隆全长基因的、限制性内切酶介导的重叠延伸法 。
METHODS IL_3 signal sequence and human endostatin gene were amplified with PCR and spliced with overlap extension PCR.
方法用PCR方法扩增il_3信号肽序列和人内皮抑素基因，然后采用重叠延伸pcr拼接的方法将两者拼接和扩增。
The common overlap extension PCR was improved and the new methods named as one-step overlap extension PCR, which can reduce the PCR times and improve the efficiency.
对常规重叠延伸PCR技术进行了改进，改进后的方法称之为一步法重叠延伸pcr，减少了进型PCR反应的次数，提高了效率。
Informix will not allow the creation or extension of a chunk to overlap an existing chunk in the same instance, whether the operation is performed manually or automatically.
Informix将不允许创建或扩展块来重叠同一实例中的现有块，不管是手动还是自动执行操作。
As the extension of rough set, variable precision rough set reflects the difference of degrees of overlap of kinds and sets.
而可变精度粗糙集作为对经典粗糙集理论的扩展，体现了等价类与集合的重叠度程度上的差别。
As the extension of rough set, variable precision rough set reflects the difference of degrees of overlap of kinds and sets.
而可变精度粗糙集作为对经典粗糙集理论的扩展，体现了等价类与集合的重叠度程度上的差别。
应用推荐