One step nested reverse transcriptase-polymerase chain reaction (RT-PCR) was used.
使用一步法巢式逆转录聚合酶链扩增法(RT - PCR)。
Conclusion: detection of BCR-ABL fusion gene by one step RT-PCR has excellent sensitivity and specificity. The method is simple and rapid, and is exactly used to clinic detection.
结论：一步法rt - P CR检测BCR - ABL融合基因有很好的灵敏度和特异性，操作简便、快速，特别适用于临床检测。
Conclusion: detection of BCR-ABL fusion gene by one step RT-PCR has excellent sensitivity and specificity. The method is simple and rapid, and is exactly used to clinic detection.
结论：一步法rt - P CR检测BCR - ABL融合基因有很好的灵敏度和特异性，操作简便、快速，特别适用于临床检测。
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