Objective: to study and establish an allelic specific primer polymerase chain reaction (ASP-PCR) technique system and to apply this technique to study single nucleotide polymorphism (SNP) of genes.
目的：研究建立等位基因特异性引物pcr技术体系，并将其应用于基因单核苷酸多态性研究工作。
A new effective single nucleotide polymorphism (SNP) typing method was developed by using real-time polymerase chain reaction (PCR).
建立一种利用荧光定量pcr扩增反应进行单核苷酸多态性(snp)快速测定的方法。
The nucleotide sequence analyses indicated that the cloned genes coded the variable light and heavy chain domains from mouse antibodies respectively.
核苷酸序列分析表明所克隆基因分别为抗体轻、重链可变区基因。
The result of the nucleotide sequence determined by dideoxy chain-termination method indicated that 5 '-terminal nucleotide sequence of the amplified NS1 fragment was not changed.
用双脱氧链末端终止法测序结果表明，NS 1基因扩增片段5端的部分碱基序列并未发生改变。
The positive recombinant plasmid was identified by PCR, enzyme digestion. The nucleotide sequence of CSP3 'ending gene was determined by the dideoxy chain termination method.
阳性重组质粒经pcr、酶切鉴定，用双脱氧链末端终止法进行序列测定。
The result of the nucleotide sequence determined by dideoxy chain-termination method indicated that 5'-terminal nucleotide sequence of the amplified NS1 fragment was not …
用双脱氧链末端终止法测序结果表明，NS1基因扩增片段5’端的部分碱基序列并未发生改变。
The result of the nucleotide sequence determined by dideoxy chain-termination method indicated that 5'-terminal nucleotide sequence of the amplified NS1 fragment was not …
用双脱氧链末端终止法测序结果表明，NS1基因扩增片段5’端的部分碱基序列并未发生改变。
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