Methods:HGV RNA was detected by a semi nested RT PCR.

方法：通过半巢式逆转录多聚酶链反应检测HGV。

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A specific semi-nested RT-PCR based on the RNA-dependent RNA polymerase gene in AF469603 of Chinese bee (Apis sinensis) sacbrood virus was established to detect Chinese bee sacbrood virus.

基于中蜂囊状幼虫病病毒基因组序列AF469603中的RNA依赖的RNA聚合酶基因，建立该病毒的半套式RT-PCR检测方法。

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So this semi-nested RT-PCR could be used for rapid detection of the muscovy duck reovirus disease.

因此，该半套式RT-PCR可以用于番鸭呼肠孤病毒病的临床快速检测。

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So this semi-nested RT-PCR could be used for rapid detection of the muscovy duck reovirus disease.

因此，该半套式RT-PCR可以用于番鸭呼肠孤病毒病的临床快速检测。

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