Aim To detect and identify human malaria parasites by nested PCR.
目的用套式pcr系统诊断、鉴别人体疟原虫感染。
Results One DNA copy of each gene could be detected with our multiplex nested PCR system.
结果巢式多重PCR检测体系可以检测出单拷贝质粒DNA。
Objective To find the reason of causing nonspecific amplication in nested PCR and abate it.
目的探索巢式PCR非特异性扩增的产生原因及消除方法。
TTV infection in the patients was retrospectively analyzed with nested PCR detection of TTV DNA in serum.
采用套式聚合酶链反应方法对合并TTV感染情况进行回顾性研究。
Therefore, the rapid, sensitive and stable semi-nested PCR are the effective methods for detecting trace bovine and sheep derived materials in feedstuff.
该技术具有快速、灵敏和结果稳定的特点，是检测饲料中痕量牛羊源成分的一种有效方法。
Compared with the conventional PCR, semi-nested PCR appeared to be more sensitive and more practicable, thus providing a reliable method to detect M. suis.
结论本试验建立的半巢式PCR诊断方法具有特异、敏感、实用等特点，为猪附红细胞体的检测提供了一种可靠的诊断方法。
In this study, a nested PCR SSP and a direct amplification PCR SSP protocols for HLA DR genotyping were developed and were used in the selection of matched donor for sibling BMT.
该研究通过设计合成hla基因序列特异性引物，建立了HLA -DR基因分型的套式扩增和直接扩增pcr -SSP技术，并在骨髓移植配型中进行了应用。
Although using nested or semi-nested PCR can prominently increase PCR specificity, it really cannot efficiently amplify quick evolving region of DNA in our research of gene Fak56D.
虽然巢式和半巢式PCR等能显著提高扩增的特异性，而应用于高变异区的扩增结果仍是杂带多或涂抹严重，不能满足后续实验需要。
Objective To assess the feasibility of detection of HIV-1 proviral DNA on Dried Blood Spot (DBS) samples by nested-PCR assay.
目的研究干血斑(DBS)样本用于人类免疫缺陷病毒1型(HIV - 1)感染前病毒DNA基因诊断的可行性。
PCR, nested-PCR and digestion analysis of the Anti-TrxS gene from T1, T2 and T3 plants showed the foreign Anti-TrxS gene was inherited to the progenies.
对本研究所得转基因后代T1、T2和T3代进行了PCR、嵌套PCR以及嵌套PCR产物的酶切消化，结果表明目的基因完整的遗传给了后代。
PCR, nested-PCR and digestion analysis of the Anti-TrxS gene from T1, T2 and T3 plants showed the foreign Anti-TrxS gene was inherited to the progenies.
对本研究所得转基因后代T1、T2和T3代进行了PCR、嵌套PCR以及嵌套PCR产物的酶切消化，结果表明目的基因完整的遗传给了后代。
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