Aim To detect and identify human malaria parasites by nested PCR.
目的用套式pcr系统诊断、鉴别人体疟原虫感染。
Results One DNA copy of each gene could be detected with our multiplex nested PCR system.
结果巢式多重PCR检测体系可以检测出单拷贝质粒DNA。
Objective To find the reason of causing nonspecific amplication in nested PCR and abate it.
目的探索巢式PCR非特异性扩增的产生原因及消除方法。
TTV infection in the patients was retrospectively analyzed with nested PCR detection of TTV DNA in serum.
采用套式聚合酶链反应方法对合并TTV感染情况进行回顾性研究。
Result (1)It is affected to the PCR results by the quantities of templates and outer cycles in one tube nested PCR.
结果1．单管巢式PCR反应条件中的模板量及外引物循环数对检测结果的影响较大。
Results:Among 23 samples of neonatal jaundice syndrome, HCMV was found in 10 by using nested PCR and virus isolation.
结果：在2 3例新生儿肝炎综合征患儿中，10例病毒分离及套式PCR均阳性；
Therefore, the rapid, sensitive and stable semi-nested PCR are the effective methods for detecting trace bovine and sheep derived materials in feedstuff.
该技术具有快速、灵敏和结果稳定的特点，是检测饲料中痕量牛羊源成分的一种有效方法。
Compared with the conventional PCR, semi-nested PCR appeared to be more sensitive and more practicable, thus providing a reliable method to detect M. suis.
结论本试验建立的半巢式PCR诊断方法具有特异、敏感、实用等特点，为猪附红细胞体的检测提供了一种可靠的诊断方法。
In this study, a nested PCR SSP and a direct amplification PCR SSP protocols for HLA DR genotyping were developed and were used in the selection of matched donor for sibling BMT.
该研究通过设计合成hla基因序列特异性引物，建立了HLA -DR基因分型的套式扩增和直接扩增pcr -SSP技术，并在骨髓移植配型中进行了应用。
Although using nested or semi-nested PCR can prominently increase PCR specificity, it really cannot efficiently amplify quick evolving region of DNA in our research of gene Fak56D.
虽然巢式和半巢式PCR等能显著提高扩增的特异性，而应用于高变异区的扩增结果仍是杂带多或涂抹严重，不能满足后续实验需要。
DNA was extracted from microorganisms in the water at 7-week and continued hunger 2-week. Nested PCR was used to detect whether GM soybean was flow in microorganisms from cultured environment.
在投喂转基因饲料7周以后以及停止投喂饥饿2周以后分离水体中的微生物，提取其DNA，进行转基因检测。
Methods Genomic DNA was extracted from the whole blood samples collected from HIV-1 positive patient. Nested PCR was carried out and PCR products were purified and used directly for sequencing.
方法从确诊的HIV - 1感染者全血样本中提取基因组dna，经套式聚合酶链反应(PCR)扩增和测序。
One step nested reverse transcriptase-polymerase chain reaction (RT-PCR) was used.
使用一步法巢式逆转录聚合酶链扩增法(RT - PCR)。
Methods Using nested reverse transcription-PCR to analyze the expression of P57KIP2, LIT1, TSSC3 in human oocytes and preimplantation embryos.
方法应用嵌套式逆转录聚合酶链反应技术检测印记基因p57kip2、LIT1、TSSC3在人类卵母细胞及植入前胚胎的正常表达。
Methods: 37 APL patients diagnosed by FAB method were measured by cytogenetics and nested RT - PCR.
方法：采用细胞遗传学和巢式rt蛳pcr方法检测FAB法确诊的37例apl初治患者。
The linear extension of the second and three rounds of nested amplification had been listed separately in improved system, it would increase the specificity and efficiency of PCR reaction.
主要是将第二、三轮的嵌套扩增中的线性延伸单独列出，从而增加了反应的特异性和高效性。
Methods:HGV RNA was detected by a semi nested RT PCR.
方法：通过半巢式逆转录多聚酶链反应检测HGV。
Objective To study the sensitivity and specificity of single tube nested polymerase chain reaction (SN PCR) technique in detecting Mycobacterium tuberculosis DNA in paraffin embedded tissues.
目的探讨单管巢式聚合酶链反应(SNPCR)检测石蜡包埋组织结核分支杆菌dna的特异性和敏感性。
Conclusions The nested RT-PCR may be a rapid, accurate and efficient way for assisting early diagnosis of patients with SARS.
结论本研究建立的套式rt - PCR方法可作为发病早期SARS病人的辅助诊断。
Objectives To establish an nested (polymerase chain reaction) PCR analytic method with restrictive enzyme to detect human cytomegalovirus (HCMV) rapidly in clinics.
目的建立套式聚合酶链反应（PCR）加限制性酶切分析方法检测人巨细胞病毒（HCMV）。
Objective To assess the feasibility of detection of HIV-1 proviral DNA on Dried Blood Spot (DBS) samples by nested-PCR assay.
目的研究干血斑(DBS)样本用于人类免疫缺陷病毒1型(HIV - 1)感染前病毒DNA基因诊断的可行性。
Nested primers were designed to improve the sensitivity of PCR reaction.
在此基础上设计巢式PCR提高此方法的灵敏度。
So this semi-nested RT-PCR could be used for rapid detection of the muscovy duck reovirus disease.
因此，该半套式RT-PCR可以用于番鸭呼肠孤病毒病的临床快速检测。
PCR, nested-PCR and digestion analysis of the Anti-TrxS gene from T1, T2 and T3 plants showed the foreign Anti-TrxS gene was inherited to the progenies.
对本研究所得转基因后代T1、T2和T3代进行了PCR、嵌套PCR以及嵌套PCR产物的酶切消化，结果表明目的基因完整的遗传给了后代。
The specificity of two primers were verified very well by nested-PCR, and they can be used as molecular probes.
经嵌套pcr验证，这两对引物特异性良好，可作为分子探针使用。
A specific semi-nested RT-PCR based on the RNA-dependent RNA polymerase gene in AF469603 of Chinese bee (Apis sinensis) sacbrood virus was established to detect Chinese bee sacbrood virus.
基于中蜂囊状幼虫病病毒基因组序列AF469603中的RNA依赖的RNA聚合酶基因，建立该病毒的半套式RT-PCR检测方法。
METHODS SENV-D and SENV-H DNA were detected in 191 cases who were divided into 6 groups with nested-PCR.
方法采用套式聚合酶链反应（nPCR）对191例、6种不同人群血清中SENVDNA进行D亚型和H亚型分析。
METHODS SENV-D and SENV-H DNA were detected in 191 cases who were divided into 6 groups with nested-PCR.
方法采用套式聚合酶链反应（nPCR）对191例、6种不同人群血清中SENVDNA进行D亚型和H亚型分析。
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