Methods The vacuum negative pressure meter and the delayed control line were used.
方法：用真空负压表、延迟控制线路，接通电话机免提键、存储拨号键。
Immortalized human embryonic kidney cell line HEK293 and the human embryonic lung fibroblasts HELF were used as positive control and negative control, respectively.
同时以端粒酶阳性的永生化人胚肾成纤维细胞HEK293作为阳性对照，以端粒酶阴性的人胚肺成纤维细胞HELF作为阴性对照。
Figure 4 a: Amplification curves of positive and negative controls in specific test. We can see that negative control did not amplify and was a straight line.
图4A：特异性实验中的阳性和阴性对照的扩增曲线，阴性对照在图中显示为一条直线，未见扩增。
Figure 4 a: Amplification curves of positive and negative controls in specific test. We can see that negative control did not amplify and was a straight line.
图4A：特异性实验中的阳性和阴性对照的扩增曲线，阴性对照在图中显示为一条直线，未见扩增。
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