Nanopore sequencing can cope with much longer strands, which should help speed things up.
纳米孔测序还可以应付更长的链,这也将帮助加速这项工作。
They also need to determine exactly how the DNA will be decoded when it passes through the nanopore.
研究人员还需要精确的确定DNA通过纳米孔时将如何被破译。
Since the molecule is easily ionized, voltage drops across the nanopore help "pull" the DNA through.
由于分子很容易被电离,通过纳米孔的电压落差有助于牵引dna穿过微孔。
This also involves a nanopore in an AHL protein, but the DNA strand does not pass through the nanopore intact.
这也需要在AHL蛋白质上有个孔道,但是DNA链不再原原本本地穿过纳米孔。
The most promising way to make such cheap, rapid sequencing a reality is an approach called “nanopore sequencing”.
让这种既便宜、又快速的测序工作成为可能最有希望的途径,是一种称作“纳米孔测序”的办法。
A detector operably coupled to a nanopore may detect labeled amino acid residues as they pass through the nanopore.
当被标记的氨基酸通过纳米孔时,与纳米孔可操作地相连的检测器可以检测它们。
Researchers took a 200-millimeter silicon-wafer chip and drilled a 3-nanometer-wide hole (known as a nanopore) through it.
研究人员那一片200毫米的硅片芯片并在上面打上直径3纳米的孔(称为纳米孔)穿过它。
Currently, the most commonly studied nanochannel is a silicon nanopore, made by drilling a hole through a silicon membrane.
目前,最常见纳米通道的研究是通过在硅表面钻了一个洞的硅纳米孔。
Scientists have shown that an applied electric field can drive the DNA molecules through a nanopore, a tiny hole in a membrane.
科学家们过去已经展示过,外加电场可以用来推动DNA分子通过纳米孔,即细胞膜上的微孔。
In the journal Nanotechnology, the physicists report the first experiment to move DNA through a solid-state nanopore using magnets.
在纳米技术杂志里,物理学家们报告了首次使用磁铁移动DNA通过固态纳米孔的试验。
There is a clear sense that the field of nanopore sequencing is gaining momentum, with new developments occurring almost every month.
毋庸置疑的是,纳米孔测序领域正在一天天地发展壮大,几乎每个月都有新的改进诞生。
Yet even a nanopore reading 50 bases per second would take about two years to sequence a whole genome, which contains around 3 billion genetic letters.
然而即使一个纳米孔每秒读取50个碱基,这仍要花费约两年时间来测序整个基因组——大约30亿个碱基。
Nanopore sequencing is expected to be much cheaper because it gets around the main problem that has kept the cost of conventional methods so high.
由于纳米孔测序客服了传统方法中最耗费开支的技术问题,所以会廉价很多。
The idea is that individual base pairs (the four chemical "letters" of the DNA alphabet) can then be read off one at a time as they pass through the nanopore.
这个想法是单个的碱基对(DNA密码的四个化学字母)在他们通过纳米孔的时候,能流利地被一个一个读出(那四个字母是指a、T、C、G,译者注)。
Dr Sanghera says Oxford Nanopore has developed an electronic cartridge system called GridION, based on "lab on a chip" technology, for exonuclease sequencing.
桑赫拉博士说“牛津纳米孔公司”已经发明出了一个基于“片上实验室”技术,叫做“网离子”的电子模块系统,用来核酸外切酶的测序。
Using self-consistent field calculation, the new equilibrium morphology of a diblock copolymer melt confined in a brush-covered cylindrical nanopore can be found.
利用自洽场方法研究两嵌段共聚物受限于接枝均聚物链(聚合物刷)圆孔中的自组装相形貌。
It would also be more accurate: because the bases are still attached to one another, there is no chance that they will pass through the nanopore in the wrong order.
这会比核酸外切酶测序更快,也会更加精确:因为碱基彼此间仍然相连,不会以错误的顺序通过纳米孔。
Other labs have experimented with similar nanopore-based approaches to sequencing but have found it difficult to control how quickly the DNA strand feeds through the nanoscale hole.
其他实验室已经试验过类似的纳米孔测序方法,但研究人员发现,很难控制DNA链穿过纳米孔的速度。
Objective To observe the growing shape and function of Madin-Darby canine kidney (MDCK) cells implanted on the polysilicon nanopore membrane by micro-electro-mechanical system (MEMS).
目的 利用多晶硅纳米孔技术人工合成一种多孔隙薄膜,并在薄膜上培养狗肾脏细胞(MDCK),观察细胞生长情况及膜的生物相容性。
It is a time-consuming process and a significant bottleneck, says Gordon Sanghera, the chief executive of Oxford nanopore, a firm set up in 2005 to commercialise Dr Bayley's work on nanopores.
这是一个很耗时的过程并且是一个重要控制步骤,戈登•桑赫拉说道。他是牛津纳米孔公司的行政长官。这家公司创立于2005年,以使贝利博士在纳米孔上的工作商业化。
So Oxford Nanopore has also been looking at an alternative approach, called exonuclease sequencing, in conjunction with Illumina, an American firm that is the market leader in rapid sequencing.
所以“牛津纳米孔公司”,和快速测序领域的领头羊,“Illumina公司”也在一同研究一种叫做“核酸外切酶测序”的办法。
The specimen for plane-view observation was prepared by peeling nanopore template film off the AAO template, widening and broking, and the broken film was put on the copper grid for observation.
纳米孔薄膜的正面观察是把纳米孔膜从模板上取下后通孔并碎化,把微小碎片放在铜网上观察;
The specimen for plane-view observation was prepared by peeling nanopore template film off the AAO template, widening and broking, and the broken film was put on the copper grid for observation.
纳米孔薄膜的正面观察是把纳米孔膜从模板上取下后通孔并碎化,把微小碎片放在铜网上观察;
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