Aim To evaluate the effect of multiplex PCR for malaria surveillance in southern Fujian Province.
目的评价复式PCR在闽南疟疾监测中的应用效果。
The multiplex PCR is a progressing rapid detection method developed from traditional PCR detection technique.
多重PCR方法是建立在传统PCR检测技术基础上的快速简便的检测手段。
Multiplex PCR which can simultaneously detect more than one bacteria has been applied increasingly and widely.
多重pcr作为一种可同时检测多种病原细菌的方法应用日益广泛。
The results indicated that the multiplex PCR technique could be applied to detect the SARS virus, successfully.
结果表明:多重pcr技术可成功应用于SARS病毒的检测。
The DNA reference targets for hybridization and subsequent on chip minisequencing was generated by multiplex PCR.
多重PCR法扩增待杂交并于芯片上微型测序的靶DNA。
Results The aim gene fragment can be amplified by a multiplex PCR assay, it largely reduce the time of identification.
结果运用群特异多重PCR法可特异性的扩增出当前流行群副溶血性弧菌的目的基因片段,可大大缩短鉴定时间。
Objective: TO establish a multiple polymerase chain reaction (multiplex PCR) technique for detection of TORCH infection.
目的:建立先天致畸多种病原体(TORCH)感染的多重pcr诊断方法。
Some scholars have established a PCR for testing PRRSV, PCV2 and CSFV, but the multiplex PCR for the three viruses is none.
有学者建立了检测prrsv、PCV2和CSFV的PCR技术,但同时检测这三种病毒的多重pcr方法还未见报道。
Objective To study the fetal DNA in maternal plasma using multiplex PCR amplification of the short tandem repent (STR) systems.
目的采用短串联重复序列(STR)多态位点的复合扩增方法,研究孕妇血浆中胎儿DNA基因型。
Multiplex PCR and real-time PCR are developed for simultaneous and quantitative detection of bacteria-producing biogenic amine.
利用多重PCR和实时定量PCR技术可以实现对葡萄酒中生物胺产生菌的快速定量检测。
Objective to design a multiplex PCR for simultaneous identification and toxigenic type characterization of Clostridium difficile isolates.
目的建立可同时进行艰难梭菌分离株菌种鉴定和毒素检测的多重pcr方法。
We developed an easy-to-use method for genotyping Toxoplasma gondii isolates in a single multiplex PCR assay with 15 microsatellite markers.
我们建立了1种易于使用的于含15种染色体微卫星标志的多重PCR中对鼠弓形虫进行分离株基因分型的方法。
Methods:Mutations of multiple genes from 106 patients with SCC were analyzed by two multiplex PCR-SSCP systems, PCR-RFLP, and DNA sequencing.
方法:对10 6例散发性大肠癌患者进行多基因突变和肠道内环境中有关指标(以粪便为标本)的测定,并进行流行病学的病例-病例研究分析。
It is revealed in this study that the multiplex PCR, specific, sensitive, rapid, can be applied to routine test of imported fish meal, meat and bone meal.
本研究表明,复合pcr是一种特异、敏感、快速的沙门菌检测方法,可应用于口岸系统鱼粉、肉骨粉的日常检测。
Abstract : Objective To establish a multiplex PCR assay for the rapid detection of pandemic group of Vibrio parahaemolyticus and toxic genes based on GS-PCR.
摘要 :目的基于多重聚合酶链反应(PCR)建立一种快速方便的检测副溶血弧菌“大流行菌群”及其毒力基因的方法。
Objective In order to set up rapid identification for the pandemic vibrio parahaemolyticus, develope a pandemic group-specific multiplex PCR assay for base unit.
前言:目的建立适于基层单位应用的针对副溶血性弧菌流行群的特异多重PCR方法。
The mosquitoes were firstly identified morphologically, and then Anopheles minimus A and C, An. aconitus, and An. jeyporiensis were identified by using multiplex PCR.
将捕获的蚊虫以传统方法进行形态学鉴定,然后用复合PCR法鉴别微小按蚊、乌头按蚊和杰普按蚊。
At present, the com-mon methods include multiplex PCR, Real Time Quantitative PCR, in situ PCR and immuno-polymerase chain reaction. They have different principles, advantages and disadvantages.
目前常用的PCR法有多重PCR技术、实时荧光定量PCR技术、原位PCR技术及免疫PCR技术,各种技术有不同的原理及优缺点。
In addition to supporting conventional applications for PCR, hybridization and sequencing, NoePrimer provides advanced capabilities for multiplex PCR and high-throughput primer search and analysis.
它不但能进行常规PCR引物、杂交探针和测序引物的设计,同时,它还能进行多重pcr引物分析以及高通量的引物搜索和查找功能。
Current multiplex polymerase chain reaction (PCR) techniques can at most offer detection from among 50 organisms in one test.
现行的多元聚合酶链反应(PCR)技术最多只能在50种生物内进行一次检测。
METHODS: Method of multiplex polymerase chain reaction (PCR) was adopted. Cyclin D1 gene and P16 gene were amplified in the same tube, using extracted genome DNA as template.
方法:采用多重聚合酶链反应方法,以提取的基因组d NA为模板,在同一反应管中同时扩增细胞周期素d 1基因和P 16基因。
Objective to investigate the correlation between phenotypic confirmatory by double disc test and genotype of CTX-M-enzymes detected by multiplex polymerase chain reaction (PCR).
目的了解采用双纸片协同法进行CTX - M酶的表型检测与多重pcr法检测基因型的相关性。
Objective To study the value of multiplex polymerase chain reaction (PCR) in diagnosing herpes virus infection of eye bank cornea donor.
目的应用多引物pcr方法快速诊断眼库供体角膜疱疹病毒感染,探讨角膜移植术后移植衰竭和疱疹病毒感染的关系。
Method the canine multiplex system were used for PCR reaction and the product were analyzed by ABI310 genetic analyzer.
方法用建立的犬复合扩增体系进行PCR反应,用abi310型遗传分析仪对扩增产物进行检测。
United application of STR linkage analysis and multiplex allele specific PCR (MASPCR) in PKU genetic diagnosis was also analysed.
同时分析了STR多态性与多重等位基因特异pcr (MASPCR)在PKU基因诊断中的联合应用。
A multiplex RT-PCR was optimized to simultaneously detect foot-and-mouth disease virus (FMDV), swine vesicular disease virus (SVDV) and vesicular stomatitis virus (VSV).
建立了一种同时检测猪口蹄疫病毒(FMDV)、猪水泡病病毒(SVDV)和猪水疱性口炎病毒(VSV)三种病原体的多重rt - PCR方法。
Results One DNA copy of each gene could be detected with our multiplex nested PCR system.
结果巢式多重PCR检测体系可以检测出单拷贝质粒DNA。
Results One DNA copy of each gene could be detected with our multiplex nested PCR system.
结果巢式多重PCR检测体系可以检测出单拷贝质粒DNA。
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