The frequencies of GSTM1-null and GSTT1-null genotypes in colorectal tumor and control groups were ascertained by using polymerase chain reaction (PCR) and multiple-PCR techniques.
应用聚合酶链式反应(PCR)、多重PCR方法检测GSTM1和GSTT1在正常人群和结直肠肿瘤患者群体中的基因多态性分布。
Methods The genotypes of GSTM1 and GSTT1 of 63 cases with HCC and 88 controls were detected with multiple PCR technique.
方法应用多重PCR技术检测63例肝癌患者和88例健康对照的GSTM1和GSTT1空白基因型。
Design and search primers for multiple applications including PCR, DNA hybridization and sequencing.
设计和搜索多重引物,包括PCR引物、序列探针和测序引物。
Then this multiple PCR indicated that it was fit for the studies on the epidemiology investigation of E. coli, Salmonella bacillus and Staphylococcus which has good sensitivity and Specificity.
进而说明该研究建立的大肠杆菌、沙门氏菌和葡萄球菌多重pcr检测方法敏感性高、特异性强,适用于大肠杆菌、沙门氏菌和葡萄球菌的流行病学调查研究。
Objective: TO establish a multiple polymerase chain reaction (multiplex PCR) technique for detection of TORCH infection.
目的:建立先天致畸多种病原体(TORCH)感染的多重pcr诊断方法。
Methods Using multiple PCR and PCR-RFLP methods, we studied the NAT1 genotypes and its genetic polymorphisms of the peripheral blood samples from 140 Han people.
方法在140名汉族健康人的外周血中,应用聚合酶链反应(PCR)限制性片段长度多态性分析(RFLP)及多重PCR技术,进行NAT1等位基因分型研究。
It also manifested that PCR-TGGE is superior to PCR-RFLP for detail dynamics of soil microbial community, and it is a rapid method to discriminate multiple samples simultaneously.
比较两种分子生物学方法的结果表明,PCR TGGE技术比pcrRFLP技术更能精确地反映土壤微生物的动态变化,并且能同时快速地对多个样品进行有效区分。
The expression of multiple gene was evaluated by DD-PCR.
荧光基因差异显示分析法检测多基因表达水平。
Methods:Mutations of multiple genes from 106 patients with SCC were analyzed by two multiplex PCR-SSCP systems, PCR-RFLP, and DNA sequencing.
方法:对10 6例散发性大肠癌患者进行多基因突变和肠道内环境中有关指标(以粪便为标本)的测定,并进行流行病学的病例-病例研究分析。
Methods:Mutations of multiple genes from 106 patients with SCC were analyzed by two multiplex PCR-SSCP systems, PCR-RFLP, and DNA sequencing.
方法:对10 6例散发性大肠癌患者进行多基因突变和肠道内环境中有关指标(以粪便为标本)的测定,并进行流行病学的病例-病例研究分析。
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