The SD-01 strain of DPV was propagated in chicken embryo fibroblast monolayer cells.
将鸭瘟病毒分离株sd - 01在鸡胚成纤维细胞上增殖。
Results Cells growth occ urred in7of10pieces of fresh limbal tissue and formed monolayer cells in1wee k.
结果10例新鲜角膜缘上皮组织培养后，7例有上皮细胞生长，1周形成细胞单层；
Objective To evaluate the effects of human frozen thawed decidua monolayer cells on mouse embryo development in a co culture system.
目的探讨冻融的人早孕蜕膜单层细胞对小鼠胚胎发育的影响。 方法蜕膜单层细胞解冻后接种获得冻融蜕膜单层细胞；
FGF-2 has been shown to retain the differentiation potential of monolayer expanded chondrocytic cells. However, its effect on nucleus pulposus cells is not known.
在单层培养的软骨细胞扩展中，FGF-2显示出可以保持分化的潜力，但此种作用在髓核细胞中尚不明确。
Conclusions the filter with PVP-free polycarbonate membrane may be used to set up the model of RPE cells with polarized growth of monolayer and investigate the barrier function of RPE cells.
结论应用无PV P聚碳酸脂膜的微孔滤器可以建立体外rpe细胞有极性单层生长的模型，并用于屏障功能的研究。
By comparison of enzyme activitys in gel entrapment and monolayer culture, it was concluded that cells of gel entrapment can maintain these enzyme activity better than those of monolayer.
通过比较，发现凝胶包埋细胞比单层贴壁培养细胞能更好地保持各项药物代谢酶的活性。
To study the methods for isolation and culture of articular chondrocyte and to observe the phenotypic expression of the monolayer cultured cells at a high density.
探讨兔关节软骨细胞的分离、培养方法，观察单层高浓度培养时细胞表型表达情况。
Method:Primary cells of human degenerative AF were obtained through in vitro monolayer culture combined with enzyme digestion method and tissue pieces culture method.
方法结合酶消化法和组织块培养法，体外单层培养人退变纤维环原代细胞。
Methods Cultured monolayer-confluent human RPE cells were scraped with a trephine and a cotton stick, and set up the injured model of RPE cells with round scraped area.
方法利用体外培养的单层近融合期人RPE细胞，采用棉签和角膜移植用环钻做圆形细胞刮伤区，建立体外RPE细胞损伤模型。
The characteristics of polarized growth of monolayer were found in RPE cells on the surface of filter membrane;
滤膜及细胞标本切面观察结果显示，RPE细胞可在滤膜表面表现出单层有极性生长的特性；
Results: A monolayer of cultured corneal limbal epithelial cells was observed by inverted microscope in both A group and B group.
结果：倒置显微镜观察，悬浮细胞培养法A组和组织块培养法B组扩增的角膜缘上皮细胞均能在羊膜上单层生长。
Objective Human degenerative nucleus pulposus cells were cultured in monolayer, the biological action of the cells was observed.
目的通过对人退变髓核细胞的原代培养，了解其生物学特性，并将绿色荧光蛋白基因转染至细胞内。
RESULTS: Sarcoma cells migrated through endothelial cells into collagen gel, the electrical resistance of a HUVEC monolayer reduced in extravasation migration of sarcoma cells.
结果：肉瘤细胞可以穿过血管内皮细胞游走至血管外；肉瘤细胞向血管外的游走能够引起单层血管内皮细胞的电阻下降；
RESULTS: Sarcoma cells migrated through endothelial cells into collagen gel, the electrical resistance of a HUVEC monolayer reduced in extravasation migration of sarcoma cells.
结果：肉瘤细胞可以穿过血管内皮细胞游走至血管外；肉瘤细胞向血管外的游走能够引起单层血管内皮细胞的电阻下降；
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