This review focuses on the principle, method and biological applications of single molecule detection based on fluorescence.
概要介绍了单分子荧光检测的原理、方法及其在生命科学中的应用。
Single molecule detection is a kind of ultra high sensitive detection technique. It is widely used in biology, medicine, environment and so on.
单分子探测技术是一项超高灵敏度的探测技术，在生物、医学和环境等领域有着广泛的应用。
Single molecule detection has made a tremendous development since 1990's.
自上世纪 90年代以来 ，单分子检测已经取得了巨大的进步。
Recently, single molecule detection has made great progress in many science areas.
近年来 ，单分子探测在许多学科领域的研究取得了显著的进展 。
The concept and experimental method of single-molecule detection based on laser-induced fluorescence in liquid fluid have been described.
介绍用激光诱发分子荧光法探测流动液体中单个染料分子的基本原理和实验方法。
Single molecule detection provides a new set of tools for scientists to explore new frontiers in the field of chemistry and life science.
单分子检测在化学及生命科学领域具有重要意义，荧光分析是单分子检测的主要手段。
We report here briefly the principle and the device of our single-molecule detection spectrometer based on two-photon excited fluorescence.
介绍采用双光子激发荧光方法进行单分子探测的原理和自行研制的实验装置，激发光聚焦和荧光收集采用共焦方式。
We report here briefly the principle and the device of our single-molecule detection spectrometer based on two-photon excited fluorescence.
介绍采用双光子激发荧光方法进行单分子探测的原理和自行研制的实验装置，激发光聚焦和荧光收集采用共焦方式。
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