Single molecule detection has made a tremendous development since 1990's.
自上世纪 90年代以来 ，单分子检测已经取得了巨大的进步。
Recently, single molecule detection has made great progress in many science areas.
近年来 ，单分子探测在许多学科领域的研究取得了显著的进展 。
This review focuses on the principle, method and biological applications of single molecule detection based on fluorescence.
概要介绍了单分子荧光检测的原理、方法及其在生命科学中的应用。
Single molecule detection provides a new set of tools for scientists to explore new frontiers in the field of chemistry and life science.
单分子检测在化学及生命科学领域具有重要意义，荧光分析是单分子检测的主要手段。
The concept and experimental method of single-molecule detection based on laser-induced fluorescence in liquid fluid have been described.
介绍用激光诱发分子荧光法探测流动液体中单个染料分子的基本原理和实验方法。
We report here briefly the principle and the device of our single-molecule detection spectrometer based on two-photon excited fluorescence.
介绍采用双光子激发荧光方法进行单分子探测的原理和自行研制的实验装置，激发光聚焦和荧光收集采用共焦方式。
Single molecule detection is a kind of ultra high sensitive detection technique. It is widely used in biology, medicine, environment and so on.
单分子探测技术是一项超高灵敏度的探测技术，在生物、医学和环境等领域有着广泛的应用。
Detection technology and methods in food microorganism have developed from culture level to molecule level.
食品微生物检测技术和方法已由培养水平向分子水平迈进。
Especially the laser-induced fluorescence detection is the most sensitive method, because it can meet to the detection of trace component, single molecule and single cell.
尤其是激光诱导荧光检测，由于其能够满足生物样品中痕量组分的测定，甚至可以达到单分子、单细胞检测水平，而成为毛细管电泳最灵敏的检测技术。
We present some typical systems studied by single molecule fluorescence detection to demonstrate its feature, advantage, and application.
本文简介近几年单分子荧光检测研究的若干典型实例，以此展示这项技术的特点、优势及其可能的应用。
It was concluded that the AgNPs-SWNTs complexes would be powerful SERS substrate for organic molecule and biological molecular detection.
此有序的银纳米枝结构是较好的表面拉曼增强(SERS)活性基底，在有机分子和生物分子的SERS检测方面将具有一定的应用前景。
In nucleic acids and proteins detection, the use of nucleic acid hybridization, immunoreaction and small molecule-protein interaction offers desirable selectivity for the assays.
在核酸和蛋白质检测研究中，利用核酸分子杂交、免疫分析、小分子与蛋白质间的特异性亲和作用提高了检测的选择性。
The detection of a highly vibrationally pump-dumped iodine wave packet dynamics in the ground X state is numerically demonstrated by using a model I2 molecule with two Morse potential surfaces.
在数值计算中，采用I2的一个二势能面系统，得到了监测基电子态高振动激发波包的吸收光谱。
The detection of a highly vibrationally pump-dumped iodine wave packet dynamics in the ground X state is numerically demonstrated by using a model I2 molecule with two Morse potential surfaces.
在数值计算中，采用I2的一个二势能面系统，得到了监测基电子态高振动激发波包的吸收光谱。
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