A significant increase of transformation efficiency was revcaled with the homologous plasmid isolated from recipient itself since the effects of host restriction and modification werc avoided.
来自受体菌自身的同源质粒,因克服了宿主的限制一修饰性,可以极显著地提高电转化效率。
Result Restriction endonuclease digestion and sequencing showed that the modification of the sense mutation of OSCP gene can be successful.
结果酶切及测序显示对OSCP基因有义突变部分的纠正获得成功。
DNA modification with restriction-modification system is a component of microbial immune system, and DNA modification without restriction system influences phenotypes by gene regulation.
由限制-修饰系统调控的DNA修饰参与微生物的免疫防御系统,无限制-修饰系统调控的DNA修饰通过调控基因表达影响表型。
DNA modification with restriction-modification system is a component of microbial immune system, and DNA modification without restriction system influences phenotypes by gene regulation.
由限制-修饰系统调控的DNA修饰参与微生物的免疫防御系统,无限制-修饰系统调控的DNA修饰通过调控基因表达影响表型。
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