Objective To review the experience in mechanical ventilation in 6 post perfusion lung cases after open heart surgery under cardiopulmonary bypass.
目的回顾性分析6例体外循环心内直视术后并发灌注肺的机械通气治疗经验。
Methods Collagenase perfusion method and mechanical cutting method were used to isolate HSCs from rat fetal liver which were then cultivated by H-DMEM containing 10% fetal bovine serum.
方法：采用胶原酶灌注法及剪碎消化法分离大鼠胎肝干细胞，并用含10%优等胎牛血清的H -DMEM培养液培养。
Methods Collagenase perfusion method and mechanical cutting method were used to isolate HSCs from rat fetal liver which were then cultivated by H-DMEM containing 10% fetal bovine serum.
方法：采用胶原酶灌注法及剪碎消化法分离大鼠胎肝干细胞，并用含10%优等胎牛血清的H -DMEM培养液培养。
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