The effect of IGFBP-3 on the growth hormone promoter activity stimulated by triiodothyronine was determined by dual-luciferase reporter assay.
通过双荧光素酶报告基因测定法测定了IGFBP-3对由三碘甲状腺素刺激的生长激素启动子活性的影响。
Methods The method of luciferase reporter gene was used.
方法采用荧光素酶报告基因方法。
We addressed this question with the method of luciferase reporter gene.
我们用荧光素酶报告基因的方法，探讨了这个问题。
Luciferase reporter gene technique plays an important role in high throughput screening.
荧光素酶报告基因分析技术在药物的高通量筛选中起着重要的作用。
Results A series of truncated NOS1 promoter luciferase reporter vectors were constructed and identified.
结果 构建并鉴定了系列截短的NOS1 启动子萤光素酶报告基因载体；
Dual-luciferase reporter vector and mutated dual-luciferase reporter vector analysis validated that they are interacted.
双荧光素酶报 告载体和突变双荧光素酶报告载体分析证实了它们能够相互作用。
ObjectiveTo construct glypican-3 (GPC3) promoter luciferase reporter gene vector, and to analyze its transcriptional activity.
目的构建磷脂酰肌醇蛋白聚糖3 (GPC3)启动子荧光素酶报告基因载体，并验证其转录活性。
In in vitro, DHT increased the AR activity and induced the expression of luciferase reporter gene in a concentration-dependent manner.
核净未能引起荧光素酶活性值的增加，但菌核净能抑制DHT依赖的AR活性，并呈现。
Objective to study the DTA vector containing the Ewing family of tumors EWS-FLI1 binding sequence depresses the co-transferred luciferase reporter plasmid.
目的研究含尤文家族肿瘤ews FLI1结合序列的DTA载体对报告基因表达的抑制作用。
The transcriptional activity of the two splicing variants was detected with a luciferase reporter in transient transfections of human embryonic kidney293T cells.
瞬时转染人胚胎肾细胞293t，用荧光素酶报告基因检测这两种剪切形式的转录活性。
In HEK293T, Dual-Luciferase Reporter Assay System was used to evaluate the effect of E4B on TLR pathway, and co-immunoprecipitation was used to identify interactive protein.
在HEK293T细胞中，通过双荧光报告系统检测E4B对TLR信号通路的影响，并且用免疫共沉淀的方法鉴定E4B的相互作用蛋白质。
CONCLUSION: Luciferase reporter vectors regulated by HRE/CMV promoters were successfully constructed, which makes it possible to further investigate their potency of hypoxia regulation.
结论：成功构建了缺氧应答启动子的荧光素酶报告载体，为进一步研究其缺氧调控作用提供了条件。
We used a reporter gene plasmid in which firefly luciferase expression is dependent on the HCV IRES.
我们使用了依赖HCVIRES表达萤火虫荧光素酶的报告基因质粒。
The effect of MDM2 on the transcriptional activity of er was detected by the reporter gene containing estrogen responsive elements luciferase (ERE-LUC).
以含雌激素反应元件的荧光素酶(ERE -LUC)为报告基因，通过检测荧光素酶活性来确定MDM2是否对ER有转录调节因子的作用。
The effect of MDM2 on the transcriptional activity of er was detected by the reporter gene containing estrogen responsive elements luciferase (ERE-LUC).
以含雌激素反应元件的荧光素酶(ERE -LUC)为报告基因，通过检测荧光素酶活性来确定MDM2是否对ER有转录调节因子的作用。
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