Study on Antimicrobial Effects of Leaves Extracts of Lonicera japonica Thunb.
金银花叶；提取物；抑菌效果。
Objective: To study extracting progress with water for chlorogenic acid of Lonicera Japonica Thunb.
目的：优选金银花绿原酸煎煮提取的最佳工艺。
Methods Chromatography was used to analyse chlorogenic acid and amino-acid of the two varieties from Lonicera Japonica Thunb.
方法用色谱法对金银花两栽培品系氨基酸和绿原酸进行定性定量分析。
Chlorogenic acid and chemical compounds of flavones in branch of lonicera japonica Thunb were determined by spectrometric method.
采用分光光度法对金银花茎中的绿原酸及黄酮类化合物的含量进行了测定。
Aim To diagnose on varieties of Lonicera Japonica Thunb with RAPD(random amplified polymorphisms DNA) and POD(peroxidase) isozyme.
目的采用多态基因研究新技术鉴定金银花品系。
Methods RAPD and POD isozyme were used to study the diagnostics between two lines, Damaohua and Jizhuahua of Lonicera Japonica Thunb on the genetic level.
方法用随机扩增多态性DNA（RAPD）技术和过氧化物酶同工酶（POD）电泳分析技术对大毛花忍冬和鸡爪花忍冬两品系进行鉴别。
Method:Two different methods, conventional CTAB method and modified CTAB method, were applied to extract genomic DNA from leaves of Lonicera japonica Thunb.
方法：采用改良CTAB法从忍冬叶片中提取DNA，通过琼脂糖凝胶电泳、紫外分光光度法、酶切检测其质量，并与常规CTAB法比较。
At last, it has been studied to make a series of pharmaceutical antibacterial activity tests about Lonicera japonica Thunb, its leaves, stems and its serum with drug.
最后，针对金银花临床上主要用于抑菌的功效，开展了金银花、叶、茎及金银花含药血清的药理学抑菌药效试验。
At last, it has been studied to make a series of pharmaceutical antibacterial activity tests about Lonicera japonica Thunb, its leaves, stems and its serum with drug.
最后，针对金银花临床上主要用于抑菌的功效，开展了金银花、叶、茎及金银花含药血清的药理学抑菌药效试验。
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