This research will helpful for further study of NDV reverse-genetic system, and the optimization of long RT-PCR will helpful for simplify the construction of clones.
本研究为建立NDV的反向遗传操作系统奠定了基础，同时对长链RT-PCR法的条件优化和简化克隆的构建提供了参考。
Conclusion RNA can be extracted from the OCT-embedded long-stored tissues treated in 25% sucrose in PBS. And all the extracted RNA can be used to RT-PCR amplification.
结论从25%蔗糖磷酸缓冲液固定oct包埋的冰冻时间较长的组织标本中可提取组织rna，并用于进行RT - PCR扩增。
Conclusion RNA can be extracted from the OCT-embedded long-stored tissues treated in 25% sucrose in PBS. And all the extracted RNA can be used to RT-PCR amplification.
结论从25%蔗糖磷酸缓冲液固定oct包埋的冰冻时间较长的组织标本中可提取组织rna，并用于进行RT - PCR扩增。
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