This is a new clinically approach to treat total corneal limbal stem cells deficiency.
为临床上由于角膜缘干细胞缺失所致的角膜疾患的治疗提供新措施。
The lack of limbal stem cells results in many abnormalities of ocular surface function.
角膜缘干细胞缺乏将导致各种眼表功能异常。
Conclusion: Limbal stem cells autograft transplantation is efficient in treating pterygium.
结论：自体角膜缘干细胞移植治疗翼状胬肉有效。
Objective:To investigate the effect of allograft limbal stem cells transplantation for acute alkali burns.
目的：观察异体角膜干细胞移植治疗眼部早期碱性化学伤临床疗效。
With these markers complex, it is easy to distinguish limbal stem cells from other epithelial cells in cornea.
采用这些标记复合体，可将角膜缘干细胞群与其他上皮细胞区分。
Objective to investigate techniques and effects of cultivated limbal stem cells allograft to treat ocular surface disorders.
目的探讨培养的异体人角膜缘干细胞移植治疗眼表疾病的方法和疗效。
Objective: To explore the effects of transplanting limbal stem cells and amnion in the treatment of cure ocular surface diseases.
目的：探讨角膜缘干细胞移植及联合羊膜移植治疗重症眼表疾病的治疗效果。
Purpose to detect P63, K3 expression in human limbus, to approach the possibility of P63 acted as the marker of human limbal stem cells.
目的通过检测人角膜缘组织中P 63、K3蛋白表达的情况，探讨P 63蛋白作为角膜缘干细胞阳性标记的可能。
Objective to reconstruct rabbit corneal epithelium in vitro with a free-feeding layer culture system and autograft to treat limbal stem cells deficiency.
目的采用无饲养层细胞培养体系构建家兔角膜上皮组织，自体移植后观察其对角膜缘干细胞缺失的治疗效果。
Method:Autologous or allosome limbal stem cells and human amnion were used in 48 patients with severe chemical injury, eyeball adhere, recurrent pterygium.
方法：采用自体或异体角膜缘干细胞联合保存人羊膜移植治疗重症化学伤、眼球粘连、复发性胬肉。
The proliferation and refreshment of corneal epithelium depends on corneal limbal stem cells. Corneal limbal stem cell deficiency can lead to visual disorder.
角膜上皮的增生与更新依赖于角膜缘干细胞，角膜缘干细胞缺乏可导致视力障碍。
Objective to study the treatment of rabbit limbal stem cell deficiency by cultured limbal stem cells growing on amniotic membrane for autograft transplantation.
目的观察以羊膜为载体的兔角膜缘干细胞膜片移植治疗兔角膜缘干细胞缺损的效果。
CONCLUSION: The transplantation of cultural homologous genic corneal limbal stem cells can be used as an effective method to treat alkali-burned ocular surface disease.
结论：培养的同基因角膜缘干细胞移植术可作为治疗眼表烧伤、重建眼表的有效方法。
Conclusion the reconstructed rabbit corneal epithelium hi vitro with a free-feeding layer culture system could be autografted to repair total corneal limbal stem cells deficiency.
结论采用无饲养层细胞培养体系构建自体角膜上皮组织，移植后可以成功修复角膜缘干细胞缺失的角膜上皮。
Objective To evaluate the effect of transplantation for treatment of rabbit limbal alkali burn by cultured limbal stem cells of fetal rabbits growing on amniotic membrane ex vivo.
目的探讨羊膜为载体培养的胎兔角膜缘干细胞移植治疗兔角膜缘碱烧伤的效果。
Background: the ocular surface diseases are common in ophthalmology in many of which the defect or disfunction of the limbal stem cells may exist. And the therapy for them is troublesome.
研究背景：眼表疾病是眼科常见病，其中许多疾病都存在角膜缘干细胞缺陷或功能障碍，治疗也相当棘手。
AIM: to observe the clinical curative effect of two different techniques to get autografts on pterygium in corneal limbal stem cells transplantation, and compare it with traditional method.
目的：观察自体角膜缘干细胞移植术中两种不同取材技术对翼状胬肉的临床疗效，并与传统方法进行比较。
CONCLUSION: the relatively pure and undifferentiated limbal stem cells can be obtained by combining non calcium medium and low calcium medium after the basal limbal cell tissues are cultured.
结论：取用角膜缘基底组织进行培养，将无钙培养液与低钙培养基结合，可获得较纯的、未分化的角膜缘干细胞。
AIM: To study the method of obtaining relatively pure limbal stem cells in rabbits cultured in vitro, and set up the experimental foundation for clinical transplantation of limbal stem cells.
前言：目的：探讨获取较纯的体外培养的兔角膜缘干细胞的方法，为角膜缘干细胞的临床移植奠定实验基础。
Objective to investigate the therapeutic effects of autologous corneal limbal stem cells transplantation for treatment of pterygium, to approach the method to reduce postoperative recurrence rate.
目的观察胬肉切除联合角膜缘干细胞移植术治疗翼状胬肉的疗效，探讨降低翼状胬肉术后复发率的方法。
Objective: to investigate the therapeutic effects of autologous corneal limbal stem cells transplantation for treatment of pterygium, to approach the method to reduce postoperative recurrence rate.
观察胬肉切除联合角膜缘干细胞移植术治疗翼状胬肉的疗效，探讨降低翼状胬肉术后复发率的方法。
Objective To construct the model of human limbal stem cells culture in vitro and evaluate the effect of autologous limbal stem cells transplantation treating with unilateral ocular surface disease.
目的：建立人角膜缘干细胞体外培养模型；探讨单眼角膜缘干细胞缺陷患者自体角膜缘干细胞体外培养后再移植的临床疗效。
STUDY SELECTION: it is to examine initially the papers relevant with studies on histology and physiopathology of limbal stem cells and relevant with autotransplantation of cultured limbal stem cells.
资料选择：选择与角膜缘干细胞组织学、生理病理学研究的相关及自体角膜缘干细胞培养移植相关文献进行初审。
ConclusionThe superficial cells of limbus and cornea are of mature status, while limbal basal cells show some undifferentiated feature, which indicates the location of stem cells.
结论角膜及角膜缘表层上皮较为成熟分化，而角膜缘基底细胞具有未分化细胞的特征，很可能是干细胞的部位。
Objective: to study in vitro cultivated limbal epithelial cells autograft with amniotic membrane grafts for total limbal stem cell deficiency of rabbit eyes.
目的：用体外培养的角膜缘上皮细胞联合人羊膜的方法，行自体移植治疗角膜缘干细胞完全缺乏的兔眼。
The stem cells of the basal layer of the limbal epithelium have function of cell renewal and tissue regeneration, and ocular surface disorders result from lack of the stem cells.
角膜缘部上皮基底层的干细胞具有细胞更新和组织再生的功能，而眼部表面疾病则是干细胞缺乏的结果。
The stem cells of the basal layer of the limbal epithelium have function of cell renewal and tissue regeneration, and ocular surface disorders result from lack of the stem cells.
角膜缘部上皮基底层的干细胞具有细胞更新和组织再生的功能，而眼部表面疾病则是干细胞缺乏的结果。
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