Objective: To set up a rapid and simple PCR assay for Candida detection and identification by using internal tran scribed spacer (ITS) universal primers.
目的：应用内转录间隔区(its)通用引物建立一种较为快速、简便的检测和鉴定念珠菌的聚合酶链反应(PCR)方法。
The internal transcribed spacer (ITS) region was amplified with the general primer (ITS1 and ITS4).
利用通用引物（ITS1和ITS4）扩增、克隆和测序了大孢指疫霉ITS区段。
The internal transcribed spacer (ITS) region was amplified with the general primer (ITS1 and ITS4).
利用通用引物（ITS1和ITS4）扩增、克隆和测序了大孢指疫霉ITS区段。
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