We have to do in-vitro cell culture experiments first, prove it in an animal model, and then develop a molecule or protein which will affect the mutated gene product.
我们首先得做一个体外细胞培养实验，在动物模型中证明它的存在，然后开发出一种能影响突变基因产物的分子或蛋白质。
Objective: to construct cell model of spermatogonial stem cells (SSCs) with Sertoli cell layer in vitro, and study the proliferation characteristic of SSCs in vitro.
目的：建立体外研究精原干细胞与支持细胞共培养的细胞模型，探讨体外精原干细胞在支持细胞层上的增殖特点。
The establishment of PC en cell line will provide a useful model in vitro for further investigating the cellular and molecular biology of exocrine and endocrine tumors of the pancreas.
EN的建立，为深入研究内、外分泌胰腺肿瘤的各种细胞和分子生物学特性提供了一个较有价值的体外模型。
To establish a cytological model of spiral ganglion neurons in vitro and to improve cell yield and viability.
目的：在体外建立大鼠耳蜗螺旋神经节神经元培养模型，并提高其产出率及细胞活性。
Stem cells can be induced into functional liver cell in vitro, the model could serve as a vehicle to study the development of liver cell and the HBV-specific receptor research.
干细胞在体外可以诱导为具有肝细胞功能的细胞，可作为研究肝细胞分化机制，对HBV的特异性受体的研究载体。
AIM: To establish a quantitative measurement method of ECV-304 cell migration in a scratch wound model in vitro.
目的：在体外细胞划痕损伤模型中建立定量测定ecv- 30 4细胞迁移的方法。
PURPOSE: 1 To build high pressure cell culture model in vitro of Retinal Ganglion Cells(RGCs) and observe the RGCs effected by pressure .
目的：1建立高压体外培养视网膜神经节细胞模型并观察压力对视网膜神经节细胞的影响；
METHODS Based on cell culture model in vitro, MTT test was used to study the inhibitory effect of curcumin derivatives on tumor cell lines.
方法利用体外细胞培养模型，应用MTT法分析姜黄素衍生物对不同肿瘤细胞生长的影响。
METHODS:Methods of in vitro cell culture model of human primary PDLC, tetrazolium bromide reduction (MTT) assay and direct contact were used.
方法：体外细胞培养，MTT （噻唑蓝）比色法，材料直接接触法。
Objective To establish an MT-2 cell model infected by HCV in vitro.
目的建立体外丙型肝炎病毒感染的MT - 2细胞模型。
Method PC12 cell was exposed to H2O2 in vitro to establish oxidative injury model.
方法体外细胞培养，H2O2处理PC 12细胞复制氧化损伤模型。
Aim: To establish a model of ischemia cerebral cell in vitro.
目的：建立一种脑细胞缺血离体模型。
Objective: to observe the effect of nerve growth factor (NGF) on cell cycle in PC12 cells, and to establish a cell cycle model of neuron-like cell in vitro.
目的：观察神经生长因子(NGF)诱导PC 12细胞周期的变化，以建立体外神经元样细胞周期的研究模型。
Methods: S180 cell incubated in vitro was used as model. The change of proliferation of S180 cell, fatty acid and sialic acid in membrane and membrane fluidity of S180 cell were investigated.
方法：采用体外培养S180肿瘤细胞为模型，观察肿瘤细胞的增殖、细胞膜上脂肪酸和唾液酸含量的变化，以及细胞膜流动性的改变。
All the research achievements will be used to build a model of three-dimensional cell culture, in order to lay a solid foundation for culture of multifunctional organ in vitro.
所有的研究结果，将用于建立细胞三维培养模型，为将来能在体外培养出全功能的人体器官打下坚实的基础。
Methods: Using in vitro cell culture and animal model to observe the effects of SPA given by oral administration on tumor proliferation, immune function and the ability of IL1, IL2 secretion.
方法：采用体外细胞培养以及小鼠动物模型，观察spa对培养肿瘤细胞的抑制，及其对小鼠动物模型肿瘤增殖、免疫及白介素1和2产生的影响。
Methods: Using in vitro cell culture and animal model to observe the effects of SPA given by oral administration on tumor proliferation, immune function and the ability of IL1, IL2 secretion.
方法：采用体外细胞培养以及小鼠动物模型，观察spa对培养肿瘤细胞的抑制，及其对小鼠动物模型肿瘤增殖、免疫及白介素1和2产生的影响。
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