The elementary procedure of this technology contains sample disposal, protein enzyme digestion, in-situ PCR and the detection of sequence copied.
原位PCR技术的基本步骤包括标本处理、蛋白酶消化、原位PCR扩增和扩增后产物的检测。
The procedure of in-situ PCR is complex, and the pseu-positive and pseu-negative results are easy to occur.
原位PCR技术操作比较复杂，同时在操作中易出现假阳性结果和假阴性结果。
Methods RT-PCR and in situ hybridization histochemistry (ISHH) were used.
方法rt - PCR和原位杂交组织化学技术。
The immuno-in situ PCR technique was introduced and described.
本文对原位免疫PCR技术进行了介绍和讨论。
At present, the com-mon methods include multiplex PCR, Real Time Quantitative PCR, in situ PCR and immuno-polymerase chain reaction. They have different principles, advantages and disadvantages.
目前常用的PCR法有多重PCR技术、实时荧光定量PCR技术、原位PCR技术及免疫PCR技术，各种技术有不同的原理及优缺点。
The indirect in situ PCR technique may provide a feasible new tool pathological detection of Toxoplasma DNA using normal paraffin sections.
间接法原位P CR为弓形虫病的病理检测提供了一种可行的新方法。
The possible application of the indirect in situ PCR technique or detection of Toxoplasma infection in liver sections of experimentally infected mice was studied.
探讨间接法原位P CR在弓形虫感染病原学检测中的应用。
The possible application of the indirect in situ PCR technique or detection of Toxoplasma infection in liver sections of experimentally infected mice was studied.
探讨间接法原位P CR在弓形虫感染病原学检测中的应用。
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