Study Design. An experimental study to investigate the in vitro gene expression of neurotrophic factors and receptors in cultured rat spinal cord cells subjected to cyclic mechanical stretch forces.
研究设计。探讨在体外培养受到循环的机械牵张力作用的大鼠脊髓细胞神经营养因子和受体基因表达的实验研究。
Objective. To determine the expression of leptin and its functional receptor in herniated disc tissues, and to elucidate whether leptin can stimulate rat NP cells to proliferate in vitro.
目的:确定瘦素及其功能受体在脱出间盘组织中的表达,阐明瘦素是否能促进鼠np细胞在体外的增殖。
Objective To pursue the changes of cell morphology, expression of differentiation related markers, and proliferation cycles of brain tumor stem cells (BTSCs) after differentiation in vitro.
目的探讨脑肿瘤干细胞(BTSC)在体外分化过程中的细胞形态、分化相关标志物表达和增殖动力学变化,为进一步研究BTSC分化走向提供实验依据。
In this article, the latest bibliography were reviewed related to applications of detergents to in vitro expression, purification, and structural investigation of membrane proteins.
简要介绍了去垢剂在膜蛋白研究中的最新应用进展,步及去垢剂在膜蛋白离体表达、分离和纯化、以及结构研究中的应用。
Objective To construct the eukaryotic coexpression plasmid encoding carcinoembryonic antigen(CEA) and heat shock proteins 70 (HSP70), then detect the expression of the plasmid in vitro.
目的构建癌胚抗原(CEA)与热休克蛋白70(HSP70)的真核双表达质粒,并检测其在体外的表达。
AIM To investigate the expression of noradrenaline bitartrate-induced heat shock protein 70HSP70 in heart in vitro and probe into the effect of HSP70 on the function of cells in isolated rat heart.
目的:观察重酒石酸去甲肾上腺素诱导后热休克蛋白70在体外心脏中的表达,并探讨热休克蛋白70对大鼠体外心脏细胞功能的影响。
Conclusion: Expression of TTF1 in vitro can be used in the further research of interaction of TTF1 and its DNA response elements and others transcription factors.
结论:能方便地获得TTF1体外翻译蛋白,为进一步研究TTF1蛋白与相应的DNA反应元件及其他转录因子的相互作用奠定了基础。
Methods: By using GFP gene as a marker, to observe expression of human tyrosine hydroxylase type I (HTH1) gene in vitro or in brains.
方法:以绿色荧光蛋白(GFP)作为标记,观察hth_1基因在体外和脑内的表达。
Moreover, DNA automaton could also be used to regulate gene expression in vitro logically. This provided a novel method for gene function analysis.
而DNA自动机已经可以根据一定的逻辑在体外调控基因的表达,为基因功能的研究提供了一种新的方法。
Objective To investigate the effects of quercetin on cell morphology and VEGF expression of acute myeloblastic leukemia cells NB4 in vitro.
目的探讨槲皮素对NB 4细胞的形态、血管内皮生长因子(VEGF)表达的影响。
Objective: To investigate Wenyang Huoxue recipe that could induce the expression of fibronectin (FN) and laminin (LN) by rat renal fibroblasts in vitro culture.
目的:探讨纤维粘连蛋白(FN)、层粘连蛋白(LN)在体外鼠肾成纤维细胞的表达及温阳活血方的干预作用。
ObjectiveTo investigate the effect of somatostatin analogue-octreotide (oct) on expression of connective tissue growth factor (CTGF) gene of murine hepatic stellate cells (HSCs) in vitro.
目的研究生长抑素类似物奥曲肽(oct)对体外培养的大鼠肝星状细胞(HSC)中结缔组织生长因子(CTGF)基因表达的影响。
Objective: To construct the prokaryotic expression vector of human heat shock protein 70(HSP 70) and to induce the expression and purification of HSP 70 in vitro.
目的:构建人热休克蛋白70(HSP 70)的原核表达载体,诱导其表达并纯化。
Objective To clone BMP7 gene in vitro, and determine the expression of BMP7 gene in articular chondrocytes of rabbits cultivated in vitro.
目的:克隆骨形态发生蛋白bmp7基因并检测其在体外培养的兔关节软骨细胞中的表达。
Objective To study the inhibition of vibramycin on the expression of matrix metalloproteinase 2 (MMP 2) and the invasiveness of androgen independent prostatic carcinoma cell line PC 3 in vitro.
目的探讨强力霉素对雄激素非依赖型前列腺癌PC3细胞基质金属蛋白酶2 (MMP 2)表达的抑制及与体外侵袭能力的关系。
Conclusion In Vitro, high glucose suppresses human fibroblast cell proliferation and stimulates PAI-1 gene expression.
结论高糖可抑制成纤维细胞增殖,刺激PAI- 1的表达。
Objective: to detect the expression of the fusion epitopes gene of foot-and-mouth disease virus (FMDV) in vitro.
目的:在体外检测口蹄疫病毒融合表位基因的表达。
Objective to observe the expression of TFPI-2 in corneal keratocytes in vitro and investigate the experimental basis for curing corneal neovascularization with gene-therapy.
目的探讨角膜基质细胞组织因子途径抑制物2 (TFPI - 2)的表达情况,为角膜新生血管的基因治疗提供方法。
Objective: to study the alteration of protein expression pattern in astrocytes in vitro following fluid percussion injury and to find biomarkers of brain injury.
目的:研究液压冲击损伤大鼠星形胶质细胞蛋白质表达谱,探寻脑损伤生物标志蛋白。
Objective the expression of various non-collagenous proteins and collagen protein on the rat fetal calvarial osteoblast cultured in low doses fluoride condition in vitro had been studied.
目的研究低浓度氟环境对体外培养的胚胎鼠颅骨成骨细胞中的各类非胶原蛋白和胶原蛋白的表达。
AIM: To investigate DNA damage and cell stress (heat shock protein 70 expression) in human bronchi smooth muscle cells by cigarette smoke extract (CSE) in vitro.
目的:探讨香烟提取物(CSE)对人支气管平滑肌细胞DNA损伤和细胞应激(热休克蛋白70表达)的影响。
Objective: to detect the expression of CD69 molecular on activated spleen lymphocyte of H22 hepatoma bearing mice in vitro.
目的:检测h 22肝癌荷瘤鼠体外活化脾淋巴细胞CD 69分子的表达。
ABSTRACT: Objective to investigate the effects of emodin on expression of cytokines induced by lipopolysaccharide (LPS) in cultured human corneal fibroblasts in vitro.
摘要:目的观察大黄素对脂多糖(LPS)诱导的角膜基质细胞表达细胞因子的影响。
Objective: To study the effects of cyclic tensile stress on the gene expression profiles of human osteoblasts in vitro.
目的:研究周期性牵张力对人成骨细胞基因表达谱的影响。
Objective To investigate the effect of progestin on the gene expression and production of osteocalcin in vitro in fetal rat calvarial osteoblasts.
目的观察孕激素对离体成骨细胞骨钙素基因表达及其合成的影响。
In in vitro, DHT increased the AR activity and induced the expression of luciferase reporter gene in a concentration-dependent manner.
核净未能引起荧光素酶活性值的增加,但菌核净能抑制DHT依赖的AR活性,并呈现。
Conclusion BMP4 may in vitro up-regulate the cholinergic expression of the neurons in hippocampus and cerebral cortex of embryo rats.
结论BMP4可体外上调胎鼠海马及大脑皮质神经元的胆碱能表达。
It is very important to produce the active CTX in vitro by gene over-expression because of the limitation of isolating CTX from snake.
目前通过捕蛇或养蛇提取蛇毒存在局限性,所以采用基因工程生产高纯度的蛇毒蛋白成为当前及今后的研究方向。
It is very important to produce the active CTX in vitro by gene over-expression because of the limitation of isolating CTX from snake.
目前通过捕蛇或养蛇提取蛇毒存在局限性,所以采用基因工程生产高纯度的蛇毒蛋白成为当前及今后的研究方向。
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