Objective: to isolate and purify murine hematopoietic stem cell using the immunomagnetic microbeads.
目的：为了建立免疫磁珠法体外分离提纯骨髓造血干细胞的方法。
Methods Immunomagnetic beads were prepared by direct antibody coating and analysed by flow cytometry.
方法运用直接包被法制备免疫磁珠，并以流式细胞仪分析。
Conclusion Direct antibody coating of immunomagnetic beads can isolate CD4 + t lymphocyte subpopulation effectively.
结论用直接包被法制备的免疫磁珠具有较好的分离CD 4 +T淋巴细胞亚群效果。
Cells sorted by immunomagnetic beads also confirmed that the stimulation had little effect on the expression of CD4-APC.
免疫磁珠分选后的细胞亦证实培养刺激对于CD 4 - APC表达的影响很小。
Objective To isolate CD4+ T lymphocyte subpopulation by antibody-coating immunomagnetic beads and evaluate the efficacy of detective methods.
目的应用抗体包被免疫磁珠分离CD4 +T淋巴细胞亚群，并检测其效果。
Various methods of enrichment exit:fluorescent activated cell sorting, magnetic-activated cell sorting, immunomagnetic beads, density gradient.
目前国外采用较多的有荧光激活细胞分选法、磁活化细胞分选法、免疫磁珠分离法、密度梯度离心法。
An immunomagnetic separation process removes a large number of the normal cells, and the specimen is further processed to break up some of the clusters.
在免疫磁性分离过程中去除大量正常细胞，然后将标本作进一步处理，打碎一些细胞团。
CD34 + cells were isolated from fresh umbilical cord blood samples with immunomagnetic beads and seeded into the models to culture ex vivo without exogenous cytokines.
免疫磁珠分选脐血cd 34 +细胞，接种于各培养体系，无外源性细胞因子条件下进行培养。
The method is based on the the immunomagnetic beads that can be used repeatedly, the technology of investigating epitopes and the technology of synthesizing multiple antigen peptides.
本课题基于可重复使用的免疫磁珠技术，抗原表位分析预测技术，多肽合成及筛选技术，建立了一种简捷快速高效的特异抗体检测方法。
Objective To compare the reliability of immunomagnetic beads and density gradient centrifugation in purification and isolation of neutrophilic granulocytes from human peripheral blood.
目的比较免疫磁珠法和密度梯度离心法分离纯化人外周血中性粒细胞的可靠性。
The result suggested that MCMS were much more suitable for purification of aim proteins than other microspheres and showed an extensive application foreground for immunomagnetic separation.
实验结果表明，纤维素磁性微球作为性能优良的新型磁性载体，更适于目的蛋白的免疫磁性分离。
The result suggested that MCMS were much more suitable for purification of aim proteins than other microspheres and showed an extensive application foreground for immunomagnetic separation.
实验结果表明，纤维素磁性微球作为性能优良的新型磁性载体，更适于目的蛋白的免疫磁性分离。
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