Selection of the feeder layer, medium, and supplemental cytokines and their roles in establishing and maintaining human ES and EG cell lines at present are illustrated in detail systematically.
详细系统地说明了迄今为止建立人胚胎干细胞系所需要的饲养层类型、培养基组成、添加细胞因子种类及其作用；
Without feeder layer, human embryonic stem cell differentiation was observed in vitro.
撤除饲养层，观察人胚胎干细胞体外分化情况。
Without feeder layer, human embryonic stem cell differentiation was observed in vitro.
撤除饲养层，观察人胚胎干细胞体外分化情况。
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