The fluorescence images of HMME and probes are detected by LSCM.
激光扫描共聚焦显微镜(LSCM)探测HMME和探针荧光图像。
Organelle-cell fluorescence intensity ratio analysis was adopted to study the intracellular distribution of HMME.
采用细胞器-细胞荧光强度比值法，对HMME进行单细胞内分布的定性与定量研究。
Objective To analyze the principal impact factors that can affect the photobleaching rate of HMME in different solutions.
目的分析在不同溶液体系中，影响国产光敏剂血卟啉单甲醚（HMME）光漂白速率的主要因素。
With incubating time prolonging, four organelles' ability to absorb HMME is enhancing in A549 cells, especially does lysosome;
随着孵育时间的延长，A549肺癌细胞各细胞器吸收HMME能力逐渐增强，尤以溶酶体显著；
The main factors that could affect the photobleaching rate of HMME were analyzed by combining the theoretic deduction and experimental results.
结合理论推导结果与实验结果分析各因素对漂白速率的影响。
METHODS: Evaluation was performed by flow cytometry on the effects of incubating concentration and time of HMME on absorption by activated T cells.
方法：以流式细胞仪检测孵育浓度和孵育时间对活化T细胞吸收HMME的影响。
Objective: To study the subcellular photodamage sites of hematoprophyrin monomethyl ether (HMME) PDT in murine lung endothelial cells applying fluorescence probes.
目的：应用荧光探针在光动力疗法研究中检测亚细胞损伤位点。
Results: For three incubation times, HMME fluorescence has diffuse cytoplasmic distribution, with little nuclear stain and brighter perinuclear area in Golgi apparatus.
结果：三种孵育时间下，HMME荧光均呈胞浆弥散分布，且在核周附近的高尔基体灰度较高，细胞核几乎无荧光分布。
Results:Under different conditions of concentration and time, HMME in both kinds of cells mainly localizes in cytoplasm and organelles, and little is found in the nucleus;
结果：不同浓度和不同时间下，HMME在两种细胞中均主要分布于胞浆及其中各细胞器内，而在细胞核内几乎无分布；
Objective: to compare three sets of fluorescence microscopy imaging systems with each other applied in study of subcellular localization of domestic new photosensitizer HMME.
目的：对三套荧光显微成像系统在国产新型光敏剂HMME亚细胞定位研究中的应用特点及适用范围进行了比较与评价。
Objective: to compare three sets of fluorescence microscopy imaging systems with each other applied in study of subcellular localization of domestic new photosensitizer HMME.
目的：对三套荧光显微成像系统在国产新型光敏剂HMME亚细胞定位研究中的应用特点及适用范围进行了比较与评价。
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