More free sulfhydryls and higher sample concentration mean that one should consider using higher concentrations of reducing agent.
自由巯基数目越多，样品浓度越高，还原剂的用量就越大。
Results the higher concentration of the protein of each sample and the better 2-de profile of each sample protein were obtained by the three-step method than by the single-step method.
结果：三步法提取的宫颈癌组织蛋白质含量较一步法有明显的增高，2 -DE蛋白质图谱较一步法清晰、分辨率高。
CONCLUSIONS The sample solution could remain much higher concentration and maintain longer period in rabbits eyes than that of control solution.
结论：样品在结膜囊中能保持较高浓度和维持较长时间。
CONCLUSIONS The sample solution could remain much higher concentration and maintain longer period in rabbits eyes than that of control solution.
结论：样品在结膜囊中能保持较高浓度和维持较长时间。
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