The rule that you quoted is intended to cover a full-range gradient (for example, 5–100% organic solvent) and refers to the amount of volume to reequilibrate at the initial conditions.
你引用的规则是用于覆盖全范围梯度洗脱的（例如，有机溶剂从5改变到100%），并且所指的体积是以初始条件来重新平衡。
When the aqueous solvent (solvent a) is cleaned up in terms of an on-line precolumn, the purity of the organic solvent (solvent b) can be tested according to the chromatogram of the blank gradient.
当含水溶剂通过预柱在线净化后，根据空白梯度色谱图可测试有机溶剂的纯度。
The ideal model of liquid chromatography in gradient elution was solved by using the characteristic approach on the assumption of a linear solvent strength model.
基于线性溶剂强度模型，应用特征线分析的方法求解梯度洗脱模式下的理想液相色谱模型。
Method The main ingredient of the injection, Red Ginseng, was determined by HPLC and used binary solvent-delivery gradient elution to control the main component in Red Ginseng.
方法采用HPLC法，以君药红参中人参皂苷为检测指标，采用梯度洗脱的方法，控制其中红参主要成分的色谱峰。
Method The main ingredient of the injection, Red Ginseng, was determined by HPLC and used binary solvent-delivery gradient elution to control the main component in Red Ginseng.
方法采用HPLC法，以君药红参中人参皂苷为检测指标，采用梯度洗脱的方法，控制其中红参主要成分的色谱峰。
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