The extracted phenols were detected by gradient eluted mobile phase and UV wavelength programming HPLC. The precision, accuracy and detection limits of established methods were determined.
采用流动相梯度淋洗，紫外检测器波长编程的方法测定富集后的酚类化合物，并测定了方法的精密度、准确度和检测限。
At last, the isoflavone is eluted with gradient aqueous alcohol.
最后，利用梯度洗脱方法将大豆异黄酮由树脂柱上洗脱下来。
At last, the isoflavone is eluted with gradient aqueous alcohol.
最后，利用梯度洗脱方法将大豆异黄酮由树脂柱上洗脱下来。
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