In 1992 Dr Prasher also cloned GFP, so that it no longer had to be harvested from living creatures.
在1992年，普拉舍博士也克隆了“绿色荧光蛋白”(gfp)，所以它不再必须是从活着的生物体中提取。
GFP has also been used for more, um, creative purposes.
绿色荧光蛋白还具有更富创造性的用途。
Dr Yun plumped instead for a chemical called Green Flourescent Protein (GFP), the substance responsible for making certain species of jellyfish glow in the dark.
Yun博士果断放弃了传统介质，选用了绿色荧光蛋白(GFP)作为介质——这种物质能使一种生活在黑暗中的水母发光。
In 2000, French scientists spliced GFP into a white rabbit's genome; Chicago artist Eduardo Kac claimed it was his idea, though scientists later disputed that.
2000年，法国科学家将绿色荧光蛋白基因转接进兔子的基因组中；芝加哥的艺术家爱德华多·卡克(EduardoKac)宣称这是他的创意，不过后来科学家们反驳了他。
This expanded the possibilities for exploiting bioluminescence dramatically, because it meant that GFP did not have to be injected into tissue.
这样极大提高了开发生物荧光的可能性，因为这意味着“绿色荧光蛋白”不再必须被注射到组织中，取而代之的是，其基因序列可以被加入到活体的基因中去。
The story behind the discovery of GFP is one with the three Nobel Prize Laureates in the leading roles.
背后的故事绿色荧光蛋白的发现之一的三个诺贝尔奖获得者中的主导作用。
Researchers have engineered a blue fluorescent probe that is smaller than GFP and will improve the labeling of peptide-fused recombinant proteins in living cells.
研究人员已经研制出一种比绿色荧光蛋白小的蓝色荧光探针，它更容易标记活细胞中肽融合重组蛋白。
While GFP is a protein that consists of 238 amino acids, the new technique USES a small, hydrophobic, and membrane-permeable fluorescent molecule.
尽管绿色荧光蛋白是由238个氨基酸组成蛋白质，是一种微小的、疏水性膜渗透的荧光分子。
However, while perfectly specific, the large size of fluorescent proteins like GFP often disrupts the natural activity of proteins inside cells.
然而，尽管有了充分特定的应用，但如绿色荧光这样较大的荧光蛋白经常会破坏细胞中蛋白的正常活动。
By using DNA technology, researchers can now connect GFP to other interesting, but otherwise invisible, proteins.
通过使用DNA技术，研究人员现在可以连接到其他有趣的绿色荧光蛋白基因，但在其他无形，蛋白质。
The green fluorescence protein (GFP) gene recombinant virus labeling is a new method for the neuroanatomical studies.
绿色荧光蛋白（GFP）基因重组病毒标记技术是神经解剖研究的新方法。
In awarding the prize, the Royal Swedish Academy compared the impact of GFP on science to the invention of the microscope.
宣布奖项时，瑞典皇家科学院称绿色荧光蛋白在科学领域的重要性堪比显微镜的发明。
The atrazine-degrading genetically engineered microorganism(GEM) was labeled by transforming a plasmid containing green fluorescent protein(GFP) gene.
通过转化绿色荧光蛋白基因质粒，对阿特拉津降解基因工程菌进行标记。
Objective To construct saccharomyces cerevisiae expression vector with GFP as report gene.
目的构建以绿色荧光蛋白（GFP）为报告基因的酿酒酵母表达载体。
In this review, we mainly describe discovery, improvement, and application of GFP.
本文主要回顾总结了绿色荧光蛋白的发现、优化改造及其应用。
The result also professes that the MAR sequence can enhance the expression of GFP gene significantly (data was shown in another paper).
研究还显示，MAR序列能明显增强绿色荧光蛋白基因的表达能力(这一结果在另文讨论)。
Methods: By using GFP gene as a marker, to observe expression of human tyrosine hydroxylase type I (HTH1) gene in vitro or in brains.
方法：以绿色荧光蛋白(GFP)作为标记，观察hth_1基因在体外和脑内的表达。
In one of his first experiments, he coloured six individual cells in the transparent roundworm Caenorhabditis elegans with the aid of GFP.
在他的第一个实验，他的个人色彩六个细胞的透明蛔虫秀丽隐杆线虫的帮助下，绿色荧光蛋白。
Osamu Shimomura first isolated GFP from the jellyfish Aequorea Victoria, which drifts with the currents off the west coast of North America.
下村修首次分离出绿色荧光蛋白的水母水母维多利亚，这与电流漂移西海岸外北美洲。
Roger Y. Tsien contributed to our general understanding of how GFP fluoresces.
罗杰元钱捐助给我们的一般理解绿色荧光蛋白荧光。
The adipose cells were transfected with Ad-GFP at the different multiplicity of infection (MOI).
以不同感染强度的携带绿色荧光蛋白基因重组腺病毒转染诱导后的脂肪细胞。
Objective To establish a culture system for human embryonic stem cell and label the hES cells with GFP by lentivirus.
目的稳定培养人胚胎干细胞，并通过慢病毒载体对其进行绿色荧光蛋白标记。
Objective To establish a culture system for human embryonic stem cell and label the hES cells with GFP by lentivirus.
目的稳定培养人胚胎干细胞，并通过慢病毒载体对其进行绿色荧光蛋白标记。
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