Objective: To clone, sequence and analyze genome segment 1 (S1) of new isolated reovirus strains.
目的：克隆测序新分离呼肠病毒的S1全长基因组片段，并对其进行序列分析。
Method: analyse its genome segment and its coding protein, then compare with its changing of surface type for infer the reason of changing.
方法分析其基因组片段和编码的蛋白质与表型变异相比较来推断发生变异的原因。
Reovirus includes 3 serotypes. Homology of proteins encoded by S1 genome segment was less than 26% between serotypes, while that of viral proteins encoded by other 9 segments was all more than 86%.
呼肠病毒共有3个血清型，不同型之间S1片段编码蛋白的同源性小于26%，其它9个片段编码蛋白的同源性都在86%以上。
If a crop developer uses genetic engineering to delete a discrete segment of a plant genome, how much regulation does that require?
如果某种作物研发者利用基因工程手段除去基因组里的某个片段，这种情形需要何种尺度的监管？
Methods: the blood samples were collected from HFRS patients between 2000 ~ 2001, and the m segment genome of hantavirus was amplified by RT-PCR.
方法：采集2000 ~ 2001年肾综合征出血热(HFRS)患者的血液标本，通过逆转录聚合酶链反应(RT - PCR)扩增汉坦病毒M片断基因。
Methods: the blood samples were collected from HFRS patients between 2000 ~ 2001, and the m segment genome of hantavirus was amplified by RT-PCR.
方法：采集2000 ~ 2001年肾综合征出血热(HFRS)患者的血液标本，通过逆转录聚合酶链反应(RT - PCR)扩增汉坦病毒M片断基因。
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