Wu J. G., S. Y. Chen, C. H. Shi and L. J. Fan, Study on culture system in gene transformation of indica rice.
吴建国、陈双燕、石春海和樊龙江，籼稻基因转化中的组织培养系统研究。
Objective: The optimization of the conditions on resveratrol synthase gene transformation for Viola diffusa Ging.
目的：通过农杆菌介导法将白藜芦醇合酶基因转化蔓茎堇菜，并对转化条件进行优化。
The result not only put a foundation for proliferation of plantlets and gene transformation but also recovered the rules of regulation of genotypes on regeneration ability.
本研究结果不仅为槐树的试管苗生产及基因转化打下基础，而且提出了基因型对植物组织培养再生能力的调控特点和规律的认识。
This report summarized the researching status of wheat exogenous gene transformation in recent years. The prospects of its applications in wheat breeding field was also analysed.
笔者综述了近年来外源基因导入小麦的研究概况，阐述了外源基因导入的技术以及检测方法，分析了外源基因转化在小麦育种中的发展前景。
Transgenes should be genes with single copy and without marker gene, and be able to express and stable inheritable in various transgenic plants in a new gene transformation program.
新的基因转化程序要求转基因为单拷贝，不带有标记基因，并在不同的转化体中表达一致，稳定遗传。
Transgenes should be with single copy and without accessory sequence, and with consistence to express and stable genetic in various transgenic plants in a new gene transformation program.
新的转化程序要求把基因导入农艺性状优良的品种中，呈单拷贝、不带有辅助序列，并在不同的转化体中表达一致，稳定遗传。
Meanwhile, there are many problems in mulberry gene transformation, such as the difficult to establishing regeneration system, high reliability on gene type, low transformation efficiency.
同时，桑树转基因也存在着再生体系建立困难，基因型依赖性强，转化效率不高等问题。
The gene transformation by agrobacterium'plasmid is a perfect and effective method of transferring gene in the gene engineering, however, there is a little apply on the monocotyledons plants.
在植物基因工程中，农杆菌质粒介导的遗传转化是比较完善与有效的基因转移方法，但在单子叶植物上应用较少。
The application of the techniques of large_scale cell culture, somatic cell fusion and gene transformation will greatly change the traditional mode of production and breeding of sweet potato.
细胞大规模培养、体细胞融合、基因转导等技术的研究和应用，可望从根本上改变甘薯传统的生产和育种模式。
The gene transformation by agrobacterium'plasmid is a perfect and effective method of transferring gene in the gene engineering, however, there is a little applied on the monocotyledons plants.
在植物基因工程中，农杆菌质粒介导的遗传转化是比较完善与有效的基因转移方法，但在单子叶植物上应用较少。
Plant transformation is an important approach in genetic engineering breeding and identification of gene function.
植物转化是开展基因工程育种和鉴定基因功能的重要途径。
The transformation system is the basis for study of pathogenicity mechanism and functional gene of the fungi.
该转化体系的建立为研究该菌的致病机制和功能基因分析奠定了基础。
The present article tried the transformation of gene into maize ovaries by microinjection.
本文首次尝试了用离体子房注射法对玉米进行基因转化。
All these results indicate that this feedback insensitive ASA2 gene can be used as a selectable marker gene for plant transformation.
这些结果表明反馈抑制不敏感ASA2基因可以作为筛选标记基因用于大豆遗传转化。
Protoplast transformation and homologous chromosomal integration of s erythraea was an important path in changing gene involved in erythromycin biosynthesis.
糖多孢红霉菌的原生质体转化和染色体同源整合，是红霉素生物合成基因改造的重要途径。
Using the above improved genetic transformation system, we have got some transformed plants of anti-sense squalene synthase gene.
利用上述改进的青蒿遗传转化体系，我们得到了反义鲨烯合酶基因的青蒿转化植株。
The results indicated that herbicide resistant gene should be used as a selection marker gene in protoplast genetic transformation of B. bassiana.
结果表明，外源基因转化球孢白僵菌原生质体时，可以以抗除草剂基因为转化筛选标记。
The EBV latent membrane protein (LMP1) gene and nucleoantigen gene EBNA2 are virus-oncogene, which can induce malignant transformation of cells.
EBV潜伏膜蛋白基因LMP1和核抗原基因EBNA2是公认的导致细胞恶性转化的病毒癌基因。
A plant expression vector was then constructed by ligating the cloned fragment to GUS gene, used for transformation of tobacco.
将其全长片段与GUS基因连接构建植物表达载体并转化烟草。
Thus the suitable acceptance system of maize genetic transformation and transferring resistant maize virus gene (PAP) were studied.
本研究从优化玉米幼胚培养遗传转化受体系统和抗病毒基因pap的转化两个方面进行研究。
Gene targeting technology, has brought the possibility of human transformation of life.
基因靶向技术的进步，带来了人类改造生命的可能。
Incorporation of a selectable marker gene during chloroplast genetic transformation is essential to obtain transformed chloroplasts, which is the same as nuclear transformation.
与核遗传转化相同，叶绿体遗传转化研究需要有合适的选择标记作为辅助手段。
Its core areas include: QTL mapping or gene discovery, Marker assisted selection, genomic selection, Genetic engineering, Genetic transformation, Issues in Biosafety and Intellectual Property.
它的核心内容主要包括QTL作图、标记辅助育种、基因组选育、基因工程、遗传转化、生物安全及知识产权问题等。
The transcription factor gene RCBF4 can be used for plant transformation so as to improve cold tolerance and hyperhaline resistance.
该转录因子基因rcbf 4能用于植物转化，提高植物的耐冷和耐高盐的能力。
Conclusion The ALS4 and ALS9 gene may play an important role in Candida albicans transformation from yeast to germ tube and in biofilm formation in vitro.
结论：ALS4基因和ALS9基因可能在白念珠菌的酵母相向菌丝相转换和体外生物膜形成中起重要作用。
A glutathione S-transferase (GST) gene from cotton was introduced into tobacco by Agrobacturium-mediated leaf disk transformation method.
用农杆菌介导的叶盘转化法将一个来自棉花的谷胱甘肽s转移酶(GST)基因转化到烟草中。
The recent advances about laser microbeam in plant transformation of exogenous gene, key techniques and applications are introduced.
研究了微束激光转化法用于棉花感受态萌动胚转化预培养的时间、高渗液对材料的处理等。
But the same as nuclear transformation, plastid transformation also has the problems of bio-safety of antibiotic markers and development obstacles due to the overexpression of foreign gene.
但同细胞核转基因一样，叶绿体转基因也存在抗生素标记的生物安全性和外源基因的过量表达对植物正常生长发育的影响的问题。
Agrobacterium and gene gun have aroused most attention among various genetic transformation methods for exogenous gene into maize.
目前外源基因导入玉米受体细胞的方法很多，最受瞩目的是农杆菌介导法和基因枪法。
Agrobacterium and gene gun have aroused most attention among various genetic transformation methods for exogenous gene into maize.
目前外源基因导入玉米受体细胞的方法很多，最受瞩目的是农杆菌介导法和基因枪法。
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