Methods The gene fragment library of SH SY5Y cells was constructed with RD PCR technique.
方法采用RD PCR技术构建SHSY5Y细胞基因片段文库，并对每一个克隆进行测序。
Using human SRY gene primers, the gene fragment of giant panda homologous to human SRY was amplified by PCR.
本文采用人sry基因的一对引物，通过PCR扩增获得了雄性大熊猫SRY基因片段。
Results The aim gene fragment can be amplified by a multiplex PCR assay, it largely reduce the time of identification.
结果运用群特异多重PCR法可特异性的扩增出当前流行群副溶血性弧菌的目的基因片段，可大大缩短鉴定时间。
Objective To obtain the gene fragment encoding human mannan-binding lectin (MBL)-associated serine protease-2 (MASP-2).
目的获得人甘露聚糖结合凝集素相关丝氨酸蛋白酶-2（MASP-2）编码区基因。
A new gene fragment has been found in one of the isolates that gives the bacteria an increased ability to spread among humans.
在其中一株引致猩红热的甲型链球菌菌株中发现多一条基因，增强细菌的传播能力，但毒性未见提高。
Through homology analysis, it is proved that the product is the gene fragment encoding Orotidine-5'-monphosphate Decarboxylase.
经过同源性分析证明该产物为三孢布拉氏霉乳清酸核苷-5'-单磷酸脱羧酶基因片段。
Gain many clone of gene fragment on differential expression, choice 3 of them for sequencing, and blast the sequence in Genebank.
获得了很多差异片段的克隆，并从其中选择3个进行测序。
Results Respectively 2 samples were positive on specific gene fragment of 14 samples of sera and 6 samples of cerebrospinal fluid.
结果14份血清和6份脑脊液标本中分别有2份标本肠道病毒特异性基因片段扩增呈阳性。
Results:The rabbit plaque in situ hybridization revealed that the gene fragment of atherosclerotic plaques in tissue proliferation.
结果：兔斑块组织原位杂交显示该基因片段在动脉粥样硬化斑块组织中表达水平增高。
Objective To study the differential expression of gene fragment in right and left optic tecta of pigeons with removal of left retina.
目的研究在左视网膜摘除后鸽与人TMEFF 2同源基因在左右视盖的差异表达。
Objective to construct eukaryotic expression vector of antisense MBD1 gene fragment and to provide a tool for studying MBD1 gene function.
目的构建反义MBD1基因片段真核表达载体，为研究MBD1基因功能提供工具。
Sequencing confirmed sCD14 gene fragment was obtained and laid the foundation for further research on expression and biological activities.
结果表明获得了人可溶性CD 14基因片段，为下一步进行CD 14表达及生物学活性研究奠定了基础。
Objective: To clone and express the MAGE-3 gene fragment (210 ~ 623nt) for researching its biological effects on MAGE-3 positive malignant tumors.
目的克隆表达人MAGE - 3基因片段(210 ~ 623位碱基)，以便研究其对MAGE - 3阳性恶性肿瘤的生物学作用。
If a gene is active, the RNA in the sample binds to the complementary gene fragment on the slide, and can be detected under ultraviolet light as a coloured dot.
如果基因是激活的，样本中的RNA就会与相应的基因片段结合在一起，再利用紫外光观察，那些激活的基因就成为一个个被染色的斑点。
Objective To construct a recombinant plasmid containing P30 gene of Toxoplasma gondii and obtain recombinant protein Methods P30 gene fragment was amplified from genomic DNA of T.
目的构建表达P30蛋白抗原表位的重组质粒及工程菌，获得纯化的P30蛋白抗原表位，用于检测弓形虫特异性抗体。
To construct a clone p-SARS-S containing SARS coronavirus s gene fragment as the positive control of RT-PCR detect methods, which can build the basis for the expression of the gene.
构建SARS冠状病毒S基因序列克隆p - SARS - S，作为自行设计、建立的RT - p CR检测方法的阳性对照，并为该基因的表达奠定基础。
Methods RNA of virus was obtained from samples of cerebrospinal fluid and serum and then gained with specific primer of Enterovirus through RT-PCR so as to detect specific gene fragment.
方法提取患者脑脊液和血清中的病毒rna，用肠道病毒特异性引物进行套式rt - PCR扩增，检测特异性基因片段。
Conclusion the four gene fragments were amplified from known genes involved in cell proliferation and differentiation, while the one unknown gene fragment maybe from a new functional leukemia gene.
结论四个基因片段为与细胞的增殖与分化相关的已知基因，有一个基因片段可能为新的功能性的白血病基因。
They were modified with fragment of the glyceraldehyde phosphate dehydrogenase gene (GAPDH) as a loading control.
它们都用磷酸甘油醛脱氢酶基因的片段作为加载控制来进行改良。
This genetic material can be in the form of a gene, a representative of a gene or cDNA, RNA or even a small fragment of a gene.
这种基因材料可以是基因，基因替代物或cDNA、RNA甚至小的基因片段。
Conclusion The positive regulatory fragment of NHE 1 gene from human lung cancer cells was successfully cloned.
结论本实验已成功地克隆了人肺癌细胞NHE1基因调控序列中正调控序列片段。
METHODS: We analyzed the gene sequences and protein structures of BMP-1 and its related proteins, and chose a highly conserved fragment as target gene.
方法：通过分析BMP - 1类各种分子的基因序列及蛋白结构，选择其中的一个片段作为目的基因。
The vector was modified to include a heterologous intron with a synthetic splice donor engineered upstream of a fragment from intron A of the gonadotropin a subunit gene(containing a splice acceptor).
载体经修改后包括带有一合成的剪接供体的一个异种内含子， 此供体经促性腺激素a亚单位基因（含有一个剪接受体）的内含子A中某片段的上游改造。
Sequence analysis showed the amplified fragment was the TIV1 fragment of the vacuolar invertase gene.
测序结果表明，所获得的片段为番茄液泡转化酶基因TIV1的片段；
AIM: To establish the mutant of coding calcium binding fragment of the 13th exon of human thrombospondin-1 (TSP-1) gene with polymerase chain reaction (PCR) site directed mutagenesis technology.
目的：利用聚合酶链反应定点突变技术构建人血小板反应素1基因第13外显子编码钙结合域突变体。
Genome DNA was extracted from white blood cell. Polymerase chain reaction (PCR) and restriction fragment-length polymorphism (RFLP) were employed to study C-344T polymorphism of CYP11B2 gene.
酚-氯仿法从外周血中提取基因组dna，聚合酶链反应(PCR)及限制性片段长度多态性(RFLP)方法检测CYP11 B2基因C - 344t多态性。
Result shows that cloning purpose fragment has the function of promoter, and it can regulate lower reaches gene expression intensely in drought, high-salt and cold.
结果表明克隆到的目的片段具有启动子功能，并且在干旱、高盐、低温诱导胁迫下更能强烈地驱动下游基因的表达。
On the basis of comparison and analysis, the reasons of population division, mark technology, target fragment gene and problems existed are discussed.
论述了病原真菌种内群体遗传分化的原因、标记的技术、标记的基因组片段、存在的问题。
A plant expression vector was then constructed by ligating the cloned fragment to GUS gene, used for transformation of tobacco.
将其全长片段与GUS基因连接构建植物表达载体并转化烟草。
A plant expression vector was then constructed by ligating the cloned fragment to GUS gene, used for transformation of tobacco.
将其全长片段与GUS基因连接构建植物表达载体并转化烟草。
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