The PP65 protein was expressed on the surface of the T7 as head fusion protein. There were 5 to 15 copies on the each phage.
PP 65目的蛋白以融合蛋白的形式表达在T7噬菌体的头蛋白部位,每个噬菌体颗粒表面可表达5 ~15个拷贝。
Specific antibody was screened by 3 rounds of panning of phage antibody library with the fusion protein. The antigen binding activity and DNA sequences of positive clones were determined and analyzed.
以该融合蛋白为固相抗原,对噬菌体抗体库进行3轮淘筛,并对所获阳性克隆进行抗原结合活性测定和DNA序列测定。
To investigate the function of PRAK in vivo, a GST fusion protein of PRAK was used as a bait and screened through T7 phage display system.
为了研究PRAK在细胞内的确切功能,我们利用GST-PRAK作为诱饵,通过T7噬菌体展示系统进行了筛选。
To investigate the function of PRAK in vivo, a GST fusion protein of PRAK was used as a bait and screened through T7 phage display system.
为了研究PRAK在细胞内的确切功能,我们利用GST-PRAK作为诱饵,通过T7噬菌体展示系统进行了筛选。
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