Methods The gene fragment library of SH SY5Y cells was constructed with RD PCR technique.
方法采用RD PCR技术构建SHSY5Y细胞基因片段文库，并对每一个克隆进行测序。
This research has referred to the predecessor in BIBAC, TAC and in the BAC method, established an own set to be possible to transform the big fragment library to construct the technical system.
本研究在参考了前人BIBAC、TAC和BAC文库构建方法的基础上，通过改进建立了一套可转化大片段文库构建技术体系。
This research has referred to the predecessor in TAC, BIBAC and in the BAC method, established an own set to be possible to transform the big fragment library to construct the technical system.
本研究在参考了前人TAC、BIBAC和BAC文库构建方法的基础上，通过改进建立了一套可转化大片段文库构建技术体系。
This research has referred to the predecessor in TAC, BIBAC and in the BAC method, established an own set to be possible to transform the big fragment library to construct the technical system.
本研究在参考了前人TAC、BIBAC和BAC文库构建方法的基础上，通过改进建立了一套可转化大片段文库构建技术体系。
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