The genome of Foot-and-Mouth disease virus(FMDV) O/LZ isolate was cloned by RT-PCR, and sequenced.
采用RT-PCR方法对口蹄疫病毒O/LZ株基因组序列进行了分子克隆和序列测定。
To develop a sensitive and specific ELISA for detection of antibodies to the nonstructural protein of FMDV.
旨在建立一种检测口蹄疫病毒非结构蛋白抗体的敏感、特异的ELISA方法。
Objective: to detect the expression of the fusion epitopes gene of foot-and-mouth disease virus (FMDV) in vitro.
目的：在体外检测口蹄疫病毒融合表位基因的表达。
FMDV is a member of the picornavirus family. Non-enveloped FMDV has a single stranded positive-sense RNA genome.
口蹄疫病毒是小RNA病毒科口蹄疫病毒属的唯一成员，不含囊膜的病毒粒子包含有一个单链正股RNA基因组。
The L fragment of the genome of foot-and-mouth disease virus(FMDV) O/NY00 isolate was cloned by RT-PCR, and sequenced.
采用RT—PCR方法对口蹄疫病毒O/NY00株基因组L片段进行了分子克隆和序列测定。
Inoculation with C731 resulted in habitation of the virus in intestine and induction of higher titre of antibodies against FMDV and EV.
接种实验动物证明，C731可寄生于动物的肠道，能使动物产生较高滴度的抗fmdv和EV抗体。
This polyclonal antibody may lay a foundation for the further studies on the biological functions and epitopes of the 3d polymerase of FMDV.
结论制备了具有高亲和性和特异性的3d聚合酶多克隆抗体，为3d聚合酶的生物学功能和抗原表位研究奠定了基础。
In this study, the immunoreactivity of structural protein VP1 of foot-and-mouth disease virus (FMDV) type o with sera from swine vaccinated against FMDV was analyzed.
研究分析了O型口蹄疫病毒(FMDV)结构蛋白vp1与当前猪f MDV疫苗血清的免疫反应性。
A multiplex RT-PCR was optimized to simultaneously detect foot-and-mouth disease virus (FMDV), swine vesicular disease virus (SVDV) and vesicular stomatitis virus (VSV).
建立了一种同时检测猪口蹄疫病毒(FMDV)、猪水泡病病毒(SVDV)和猪水疱性口炎病毒(VSV)三种病原体的多重rt - PCR方法。
Then capture FMDV from clinical material with sterilized Eppendrof tubes of coating type-specific IgG, Amplify the conserved viral sequences by common primers (FM1/FM4).
在FMDV的核苷酸序列相对保守区设计上下游引物FM1/FM4，扩增病毒的基因片段。
The purified protein could specificially react with FMDV infection antibodies in Western blotting assay, but no reaction with the immune antibodies induced with vaccine.
能与口蹄疫病毒感染血清发生特异性反应，而不能与疫苗免疫动物血清发生反应；
Objective to developed a recombinant protein vaccine with epitopes on VP1 protein for prevention of foot-and-mouth disease virus (FMDV) spreading by inactive-FMD vaccine.
目的为了克服灭活口蹄疫病毒疫苗可能存在的传播病毒的潜在危险，构建一种能预防O型口蹄疫病毒感染的VP1表位重组蛋白疫苗。
An indirect ELISA was established with expressed 3a protein of FMDV as coating antigen and was experimentally used to detect FMDV infection sera, immune sera and other disease sera.
用表达的口蹄疫3a蛋白作为包被抗原，建立了间接elisa试验，对口蹄疫病毒感染血清、免疫血清和其它非口蹄疫病毒血清进行了检测研究。
The results show that microcalorimetric study can be applied in virus infection research through measuring metabolic energy released from BHK-21 cell and BHK-21 cell infected by FMDV.
结果表明，微量热法通过对细胞及其受病毒感染的细胞体系代谢热的测定，能有效地监测病毒在宿主细胞内增殖的过程。
The epidemic strain O/YNGM/97 in China was identified to be foot-and-mouth disease virus(FMDV) type O using serological methods, and its biological and immunological characteristics were stu-died.
利用血清学方法对我国流行毒株O/YNGM/97进行鉴定，并对其生物学特性和免疫学特性进行了研究。
The epidemic strain O/YNGM/97 in China was identified to be foot-and-mouth disease virus(FMDV) type O using serological methods, and its biological and immunological characteristics were stu-died.
利用血清学方法对我国流行毒株O/YNGM/97进行鉴定，并对其生物学特性和免疫学特性进行了研究。
应用推荐