Using fluorescence quantitative PCR method of quantitative analysis.
采用荧光定量pcr的方法进行定量分析。
Objective To evaluate the clinical value of fluorescence quantitative PCR(FQ-PCR) technique in diagnosing tuberculosis.
目的探讨荧光定量聚合酶链反应(FQ-PCR)技术诊断结核病临床应用价值。
Objective To investigate the clinical application of the Fluorescence quantitative PCR (FQ-PCR) in the detection of HCV infection.
目的:用荧光定量聚合酶链反应(FQ - PCR)测定丙型肝炎病毒在临床的应用。
Methods The serum samples from 310 cases of viral hepatitis were tested by Fluorescence quantitative PCR assay (FQ PCR), and also by ELISA as contrast.
方法运用荧光定量pcr (FQ - pcr)和el IS A两种方法同时检测了310份肝炎患者血清,并对结果进行了对比分析。
To filter out some of the differential genes, 18 cases of AS group and 6cases of healthy control group are validated by real-time fluorescence quantitative PCR.
对筛选出的部分差异基因,选取病例组18例及正常对照组6例,以实时荧光定量PCR进行验证。
Methods an Alkaline lysis method was used to isolate serum HBV-DNA for fluorescence quantitative PCR and compared with conventional phenol-chloroform extraction method and boiling lysis method.
方法使用碱裂解法、酚氯仿法和煮沸裂解法同时提取血清hbv - DNA,比较荧光定量pcr测定的重复性和测定值的差异。
Using this model to do quantitative PCR analysis, result error is only related to the accuracy of fluorescence intensity or the instrument used.
使用动力学数学模型做定量pcr分析,其结果的误差仅与使用的荧光强度数值的精确度相关。
Real-time fluorescent quantitative PCR is a kind of technique that can quantify the nucleic acid on different fluorescence, as it features high sensitivity, accuracy, and specificity.
实时荧光定量PCR技术是一种利用荧光检测方法来定量核酸的技术,具有高度的灵敏性、特异性和精确性。
Results : Detection of MTHFR gene C677T polymorphism was quickly finished by realtime fluorescence quantitative RT - PCR, and the results were proved to be effective by PCR - RFLP.
结果:荧光定量方法可以快速完成MTHFR C677T基因多态性的检测,检测结果得到传统PCR-RFLP方法的证实。
Liver function and the markers of HBV were detected. The contents of HBV- DNA in serum and in gastric mucosa were assayed respectively by fluorescence quantitative polymerase chain reaction (FQ-PCR).
用核酸扩增荧光定量法检测血清、胃黏膜HBVDNA ,综合分析各检测值对肝胃不和证积分的意义。
Objective To investigate the fluorescence quantitative polymerase chain reaction (FQ-PCR) in the giant cell viral hepatitis in value.
目的探讨荧光定量聚合酶链反应(FQ - PCR)在巨细胞病毒性肝炎中的应用价值。
Uses real-time fluorescence quota PCR the method to carry on the quantitative analysis.
采用实时荧光定量pcr的方法进行定量分析。
Uses fluorescence quota PCR the method to carry on the quantitative analysis.
采用荧光定量pcr地办法停止定量剖析。
Methods MDR1 gene expression in case of 30 leukemia and 8 healthy persons' peripheral blood have been detested by fluorescence-quantitative reverse transcription-polymerase chain reaction (RT-PCR).
方法应用荧光定量逆转录-多聚酶链反应(RT -PCR)检测了30例急性白血病患者和8例正常人外周血MDR1基因的表达。
Objective The serum HBV-DNA has been extensively detected by fluorescence-quantitative real time PCR (FQ-PCR) in our country.
目的目前国内较多的医院采用荧光定量的方法对血清中的HBV - DNA进行定量检测。
Objective The serum HBV-DNA has been extensively detected by fluorescence-quantitative real time PCR (FQ-PCR) in our country.
目的目前国内较多的医院采用荧光定量的方法对血清中的HBV - DNA进行定量检测。
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