Objective To construct recombinant plasmid with human atrial natriuretic peptide (ANP) gene in fusion form for stable and high level expression of genetic engineering product ANP in E. coli system.
目的构建以融合蛋白形式在大肠杆菌中高效表达心钠素的重组质粒，稳定高效地获得基因工程产品心钠素。
Expression products of recombinant plasmid mainly located in nucleus.
重组质粒表达产物定位于细胞核；
Objective To construct a recombinant plasmid for expression of rabies virus glycoprotein in COS-7 cells.
目的构建狂犬病病毒糖蛋白基因的真核细胞表达载体，并在COS - 7细胞中表达。
Objective To construct recombinant plasmid for expression of chymopapain in E.
目的构建木瓜凝乳蛋白酶的表达载体，并在大肠杆菌中表达。
Conlusion The human FHIT recombinant eukaryotic expression plasmid was constructed successfully.
结论成功构建了重组人FHIT真核表达质粒。
Conclusion The recombinant expression plasmid constructed by restriction enzyme cleave identification can highly express recombinant human ZP3 protein.
结论：经酶切鉴定构建的人透明带蛋白3重组表达载体可高效表达重组人zp3蛋白。
Supercoiling is necessary for the expression of recombinant plasmid DNA at transcription level.
超螺旋化对于质粒dna在转录水平上的表达是必需的。
After the reading frame was confirmed correct by sequencing, the recombinant plasmid was transformed into expression host e.
测序表明读码框架正确后，将重组子分别转入表达宿主菌e。
CONCLUSION: a recombinant eukaryotic expression plasmid of amastin gene of Leishmania Donovani was successfully constructed, and can be expressed stably in the NIH3T3 cells.
结论：成功地构建杜氏利什曼原虫无鞭毛体蛋白基因的真核表达重组质粒，并且该基因在NIH3T3细胞中获得了稳定表达。
CONCLUSION: a recombinant eukaryotic expression plasmid of amastin gene of Leishmania Donovani was successfully constructed, and can be expressed stably in the NIH3T3 cells.
结论：成功地构建杜氏利什曼原虫无鞭毛体蛋白基因的真核表达重组质粒，并且该基因在NIH3T3细胞中获得了稳定表达。
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